BACTERIAL SPORE COMPOSITIONS FOR INDUSTRIAL USES

• US 20160040119A1
• Filed: 08/06/2015
• Published: 02/11/2016
• Est. Priority Date: 08/06/2014
• Status: Active Grant

• Current Assignee: Envera LIC, LLC
• Original Assignee: Envera, Llc (Intercontinental Exchange, Inc.)
• Inventors: Hashman, Tommie Eugene
• Granted Patent: US 9,932,543 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A01N 25/00 Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application , e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• CPC Class Codes: A01N 37/44 containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
• CPC Class Codes: A01N 37/46 N-acyl derivatives
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A01N 59/00 Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
• CPC Class Codes: A01N 63/22 Bacillus
• CPC Class Codes: A01N 63/28 Streptomyces
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A61K 2035/115 Probiotics
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 47/12 Carboxylic acids; Salts or anhydrides thereof
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: B09C 1/10 : microbiologically, biologically or by using enzymes
• CPC Class Codes: B09C 1/105 : using fungi or plants
• CPC Class Codes: B09C 2101/00 : In situ
• CPC Class Codes: C02F 11/04 : Anaerobic treatment; Production of methane by such processes
• CPC Class Codes: C02F 3/34 : characterised by the microorganisms used
• CPC Class Codes: C02F 3/348 : characterised by the way or the form in which the microorganisms are added or dosed
• CPC Class Codes: C05F 11/08 : Organic fertilisers containing added bacterial cultures, mycelia or the like
• CPC Class Codes: C09K 8/582 : characterised by the use of bacteria
• CPC Class Codes: C11D 3/221 : Mono, di- or trisaccharides or derivatives thereof
• CPC Class Codes: C11D 3/33 : Amino carboxylic acids
• CPC Class Codes: C11D 3/381 : Microorganisms
• CPC Class Codes: C12N 1/20 : Bacteria; Culture media therefor
• CPC Class Codes: C12P 5/023 : Methane
• CPC Class Codes: Y02A 40/818 : Alternative feeds for fish, e.g. in aquacultures
• CPC Class Codes: Y02E 50/30 : Fuel from waste, e.g. synthetic alcohol or diesel
• CPC Class Codes: Y02W 10/20 : Sludge processing
• CPC Class Codes: Y02W 30/40 : Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.

Claims

1. A composition comprising a bacterial spore and a germinative compound.

2. The composition of claim 1, wherein the spore and the germinative compound are maintained in an inactive form such that the germinative compound will not induce germination of the spore until the composition is subjected to an activation environment.

3. The composition of claim 1, wherein the composition further comprises a substance which prohibits the bacterial spore and the germinative compound from interacting until the composition is subjected to an activation environment.

4. The composition of claim 3, wherein the substance is selected from the group consisting of a surfactant, a sequestering agent, a plasticizer, a colorant, a dye, a brightener, an emulsifier, a flow agent, a coalescing agent, a defoaming agent, a thickener, a wax, a bactericide, a filler, a polymer, a wetting agent, and an anti-freezing agent.

5. The composition of claim 1, wherein the composition comprises an intimate mixture of the bacterial spore and the germinative compound, wherein the bacterial spore and the germinative compound are maintained in proximate position until they reach an environment conducive to germination.

6. The composition of claim 1, wherein the bacterial spore is selected from the group consisting of Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus altitudinis, Bacillus amyloliquefaciens, Bacillus butanolivorans, Bacillus cereus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus firmus, Bacillus kurstaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus licheniformis, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus mojavensis Bacillus mycoides, Bacillus natto, Bacillus nigrificans, Bacillus popillae, Bacillus pumilus, Bacillus siamensis, Bacillus simplex, Bacillus sphaericus, Bacillus spp., Bacillus subtilis, Bacillus thuringiensis, Bacillus unifagellatus, Clostridium thermocellum, Clostridium ljungdahlii, Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium butyricum, Pasteuria penetrans, Pasteuria thornei, Pasteuria nishizawae and Streptomyces species.

7. The composition of claim 1, wherein the germinative compound is selected from the group consisting of fructose, glucose, potassium, L-alanine, L-valine, L-proline, L-leucine, L-cysteine, L-threonine, L-glutamine, L-asparagine, L-phenylalanine and analogues thereof.

8. The composition of claim 1, wherein the bacterial spore is selected from the group consisting of B. subtilis, B. amyloliquefaciens, B. firmus, B. licheniformis, B. megaterium, and B. pumilus;

and the germinative compound is selected from the group consisting of L-alanine, L-valine and L-asparagine.

9. The composition of claim 1, wherein the composition is in the form of a dry powder.

10. The composition of claim 1, wherein the composition is in the form of an emulsion.

11. A method for treating wastewater comprising adding the composition of claim 1 to wastewater.

12. A method for environmental remediation comprising applying the composition of claim 1 to soil or water.

13. A method for treating water in an aquaculture system, comprising contacting the composition of claim 1 with water in an aquaculture system.

14. A method for feeding an animal in an aquaculture system, comprising administering an animal feed comprising the composition of claim 1 to the animal in the aquaculture system.

15. A probiotic comprising the composition of claim 1 and an acceptable carrier.

16. A method of preparing a probiotic, comprising mixing the composition of claim 1 with an acceptable carrier.

17. A method for feeding an animal comprising administering the composition of claim 1 to an animal.

18. A direct fed microbial comprising the composition of claim 1 and an acceptable carrier.

19. A method of preparing a direct fed microbial comprising mixing the composition of claim 1 with an acceptable carrier.

20. A cleaning product comprising the composition of claim 1 and an acceptable carrier.

21. A method of preparing a cleaning product comprising mixing the composition of claim 1 with an acceptable carrier.

22. A method of producing methane from waste material, comprising applying the composition of claim 1 to waste material.

23. A method of treating animal waste, bedding or litter comprising applying the composition of claim 1 to animal waste, bedding or litter.

24. A method of preparing silage comprising applying the composition of claim 1 to silage.

25. A method for microbial enhanced oil recovery (MEOR), comprising applying the composition of claim 1 to an oil well.

26. A method of treating or preventing a disease in a subject susceptible to or afflicted with the disease comprising administering to the subject an effective amount of the composition of claim 1, thereby treating or preventing the disease in the subject.