COMPOSITIONS AND METHODS OF NUCLEIC ACID-TARGETING NUCLEIC ACIDS
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Abstract
This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.
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Citations
43 Claims
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1-26. -26. (canceled)
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27. An engineered nucleic acid targeting nucleic acid, comprising:
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an engineered CRISPR RNA (crRNA), an engineered trans-activating CRISPR RNA (tracrRNA), or a combination thereof, wherein (i) the engineered nucleic acid targeting nucleic acid can associate with a Cas9 protein to form a nucleic acid targeting nucleic acid-Cas9 complex capable of binding a target DNA, and (ii) the engineered crRNA, the engineered tracrRNA, or the combination thereof is altered relative to a corresponding wild-type crRNA, tracrRNA, or both; and the engineered nucleic acid targeting nucleic acid comprises at least one of the following;
a duplex variant, a spacer extension, a mid-tracr modification, or a tracr extension. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification