COMPOSITIONS AND METHODS OF NUCLEIC ACID-TARGETING NUCLEIC ACIDS
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Abstract
This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.
72 Citations
40 Claims
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1-26. -26. (canceled)
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27. A composition comprising:
a modified Cas9 protein, wherein the modified Cas9 protein comprises a modification of a region of a wild-type Cas9 protein selected from the group consisting of a Cas9 bridge helix region;
a Cas9 highly basic patch within the Cas9 N-terminal region;
a Cas9 HNH domain region;
a Cas9 RuvC domain region;
a Cas9 P-domain recognition region;
an added enzymatic activity;
insertion, deletion or substitution of a domain;
insertion, deletion or substitution of a structural motif;
a fusion; and
combinations thereof.- View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
Specification