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METHODS AND APPARATUS FOR SYNTHESIZING NUCLEIC ACID

  • US 20160046973A1
  • Filed: 08/18/2015
  • Published: 02/18/2016
  • Est. Priority Date: 04/02/2013
  • Status: Active Grant
First Claim
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1. A method for synthesizing an oligonucleotide, comprising:

  • exposing an oligonucleotide attached to a solid support to a nucleotide analog in the presence of a nucleotidyl transferase enzyme and in the absence of a nucleic acid template such that the nucleotide analog is incorporated into the oligonucleotide,wherein the nucleotide analog comprises a nucleotide coupled, by a cleavable linker, to an inhibitor that sterically prevents the nucleotidyl transferase from catalyzing incorporation of a nucleotide or an additional nucleotide analog into said oligonucleotide until said inhibitor is removed by cleavage of said cleavable linker.

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