Compositions and Methods for the Transfer of a Hexosamine to a Modified Nucleotide in a Nucleic Acid
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Abstract
Nucleic acids comprising β-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.
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Citations
35 Claims
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1-18. -18. (canceled)
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19. A method, comprising:
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(a) attaching a hexosamine to a modified nucleotide in a nucleic acid fragment (b) adding a label to the hexosamine to form a labeled modified nucleotide; and (c) detecting the labeled modified nucleotide and/or enriching for the nucleic acid containing the labeled modified nucleotide. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A kit comprising a purified β
- -glucosyl transferase and a purified UDP-glucosamine in a buffer in separate or the same reaction vessel.
- 32. A nucleic acid comprising a modified nucleotide covalently attached to a hexosamine.
Specification