An image forming method of a fluorescent sample
First Claim
1. An image forming method of a fluorescent sample comprising the steps of:
- detecting fluorescent points (9, 11) of said sample by a detecting apparatus (10, 8A, 8B), obtaining therefrom detected fluorescent points; and
forming images of said detected fluorescent points on a display (12);
wherein the step of detecting comprises;
pre-defining a scanning field (2) of said sample, which comprises a set of fluorescent detectable points (9, 11);
irradiating statically in a first initial step at least a first sub-set of first points (9) of said set of detectable points by a first irradiating beam (8A) generated by an irradiation device (10);
resetting said irradiation device (10) to generate a second irradiating beam (8B) different from said first irradiating beam (8A);
irradiating statically in a subsequent irradiation step at least a second sub-set of second points (11) of said set of detectable points by said second irradiating beam (8B) generated by said reset irradiation device (10), which are different from said first points (9) and that form said set of detectable points when added to said first sub-set; and
completing an image on said display (12) by supplementing reciprocally said first sub-set and second sub-set of detected points.
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Abstract
A method of imaging a fluorescent sample includes the steps of: scanning fluorescent points of the sample using a scanner apparatus, thereby obtaining scanned fluorescent points; and imaging the scanned fluorescent points on a display, the scanning including the steps of predefining a scan field for the sample, which includes a set of scannable fluorescent points; and sequentially irradiating, at least one first subset of points of the set of points and at least one second subset of the set of points, which complements the first subset with respect to the set of points. The first and second subsets can be irradiated at different focal irradiation distances.
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Citations
6 Claims
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1. An image forming method of a fluorescent sample comprising the steps of:
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detecting fluorescent points (9, 11) of said sample by a detecting apparatus (10, 8A, 8B), obtaining therefrom detected fluorescent points; and forming images of said detected fluorescent points on a display (12); wherein the step of detecting comprises; pre-defining a scanning field (2) of said sample, which comprises a set of fluorescent detectable points (9, 11); irradiating statically in a first initial step at least a first sub-set of first points (9) of said set of detectable points by a first irradiating beam (8A) generated by an irradiation device (10); resetting said irradiation device (10) to generate a second irradiating beam (8B) different from said first irradiating beam (8A); irradiating statically in a subsequent irradiation step at least a second sub-set of second points (11) of said set of detectable points by said second irradiating beam (8B) generated by said reset irradiation device (10), which are different from said first points (9) and that form said set of detectable points when added to said first sub-set; and completing an image on said display (12) by supplementing reciprocally said first sub-set and second sub-set of detected points. - View Dependent Claims (2, 3, 4, 5)
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6. An image generating apparatus of a fluorescent sample, comprising:
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a source (5) of a detecting ray beam (4); and a spatial light modulator 810) equipped with a phase modulator, designed to modify a phase of said detecting ray beam (4) into at least a first beam (8A) of detecting sub-rays of a first sub-set of detectable points (9) and into a second beam (8B) of detecting sub-rays of a second sub-set of detectable points (11) of said sample.
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Specification