SYSTEM AND METHOD FOR ITERATIVE DETECTION OF BIOLOGICAL MOLECULES
First Claim
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1. A system for iterative detection of biological molecules, comprising:
- a probe; and
a fluorophore tethered to the probe with an azide-based linker;
wherein the linker is configured to be cleaved in the presence of tris(2-carboxyethyl)phosphine (TCEP), andwherein the on/off ratio between a signal measured before treatment with TCEP and a signal measured after treatment with TCEP is at least about 20;
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Abstract
A system for the iterative detection of biological molecules includes a probe, and a fluorophore tethered to the probe with an azide-based linker. The linker is configured to be cleaved in the presence of tris(2-carboxyethyl)phosphine (TCEP), and the on/off ratio between a signal measured before treatment with TCEP and a signal measured after treatment with TCEP is at least about 20:1.
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Citations
14 Claims
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1. A system for iterative detection of biological molecules, comprising:
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a probe; and a fluorophore tethered to the probe with an azide-based linker; wherein the linker is configured to be cleaved in the presence of tris(2-carboxyethyl)phosphine (TCEP), and wherein the on/off ratio between a signal measured before treatment with TCEP and a signal measured after treatment with TCEP is at least about 20;
1. - View Dependent Claims (2, 3, 4, 5)
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6. A method of detecting a biological molecule, comprising:
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incubating a sample including a target molecule with a detection system, the detection system including a probe and a fluorophore tethered to the probe with an azide-based linker; exciting the sample at a first wavelength; detecting the emission of a second wavelength from the sample; and treating the sample with tris(2-carboxyethyl)phosphine, thereby cleaving the fluorophore from the probe. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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Specification