SENSOR AND CONCENTRATION MEASUREMENT METHOD

• US 20160061764A1
• Filed: 11/10/2015
• Published: 03/03/2016
• Est. Priority Date: 08/31/2009
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: TAKAHARA, Yoshifumi, NAKAMINAMI, Takahiro, IKEDA, Shin
• Granted Patent: US 10,761,044 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A reagent layer of a sensor contains as a mediator a quinone compound having a hydrophilic functional group, phenanthrenequinone, and/or a phenanthrenequinone derivative. The quinone compound has a lower redox potential than a conventional mediator, so interfering substances have less effect on detection results with this sensor.

Claims

1. A sensor for detecting or quantifying a target substance contained in a liquid sample including blood, comprising:

a phenanthrenequinone derivative as a mediator;

an enzyme dehydrogenating or oxidizing the target substance;

a working electrode;

a counter electrode; and

a reagent layer including the enzyme and the phenanthrenequinone derivative,

wherein the reagent layer is disposed so as to be in contact with the working electrode and/or the counter electrode.

2. The sensor according to claim 1,

wherein the phenanthrenequinone derivative is a 9,10-phenanthrenequinone derivative.

3. The sensor according to claim 1,

wherein the phenanthrenequinone derivative has a hydrophilic functional group.

4. The sensor according to claim 3,

wherein the phenanthrenequinone derivative has at least one type of functional group selected from the group consisting of a sulfonic acid group, a carboxylic acid group, and a phosphoric acid group as the hydrophilic functional group.

5. The sensor according to claim 1,

comprising, as the phenanthrenequinone derivative, at least one type of compound selected from the group consisting of:

9,10-phenanthrenequinone-2-sulfonic acid,

9,10-phenanthrenequinone-1-sulfonic acid,

9,10-phenanthrenequinone-3-sulfonic acid,

9,10-phenanthrenequinone-4-sulfonic acid,

9,10-phenanthrenequinone-2,7-disulfonic acid,

9,10-phenanthrenequinone-2-carboxylic acid, and

9,10-phenanthrenequinone-2-phosphoric acid.

6. The sensor according to claim 1,

wherein the enzyme is a redox enzyme.

7. The sensor according to claim 6,

wherein the redox enzyme is an oxidase.

8. The sensor according to claim 7,

wherein the redox enzyme is an FAD-dependent oxidase.

9. The sensor according to claim 6,

wherein the redox enzyme is a dehydrogenase.

10. The sensor according to claim 9,

comprising at least one type of enzyme selected from the group consisting of an NAD-dependent dehydrogenase, a PQQ-dependent dehydrogenase, and an FAD-dependent dehydrogenase as the redox enzyme.

11. The sensor according to claim 10,

wherein the redox enzyme is an FAD-dependent glucose dehydrogenase.

12. The sensor according to claim 6,

wherein the redox enzyme is a PQQ-dependent or FAD-dependent enzyme.

13. The sensor according to claim 1,

wherein the amount of the phenanthrenequinone derivative contained in the sensor is 0.05 to 2500 nmol per unit of the enzyme.

14. The sensor according to claim 1,

wherein the amount of the phenanthrenequinone derivative contained in the sensor is 1 to 400 nmol per unit of the enzyme.

15. A sensor for detecting or quantifying a target substance contained in a liquid sample including blood, comprising:

a working electrode;

a counter electrode;

a quinone compound having quinone and at least one hydrophilic substituent; and

a coenzyme-dependent enzyme dehydrogenating or oxidizing the target substance;

wherein oxidation-reduction potential of the quinone compound is less than 0, and the oxidation reduction potential of the quinone compound is greater than oxidation reduction potential of the coenzyme.

16. The sensor according to claim 15,

wherein the coenzyme-dependent enzyme is a PQQ-dependent, an FAD-dependent, or an NAD-dependent enzyme.

17. The sensor according to claim 16,

wherein the PQQ-dependent, the FAD-dependent, or the NAD-dependent enzyme is a enzyme dehydrogenating the target substance.

18. The sensor according to claim 16,

wherein the FAD-dependent enzyme is a enzyme oxidizing the target substance.

19. The sensor according to claim 15,

wherein the substituent has a benzene ring and the hydrophilic functional group added to the benzene ring.

20. The sensor according to any of claim 15,

wherein the substituent has at least one type of functional group selected from the group consisting of a sulfonic acid group, a carboxylic acid group, and a phosphoric acid group.

21. The sensor according to claim 15,

wherein the quinone is a phenanthrenequinone.

22. The sensor according to claim 21,

wherein the phenanthrenequinone is a 9,10-phenanthrenequinone.

23. The sensor according to claim 22,

wherein the quinone compound has at least one type of compound selected from the group consisting of:

9,10-phenanthrenequinone-2-sulfonic acid,

9,10-phenanthrenequinone-1-sulfonic acid,

9,10-phenanthrenequinone-3-sulfonic acid,

9,10-phenanthrenequinone-4-sulfonic acid,

9,10-phenanthrenequinone-2,7-disulfonic acid,

9,10-phenanthrenequinone-2-carboxylic acid, and

9,10-phenanthrenequinone-2-phosphoric acid.