SHORT INTERFERING NUCLEIC ACID (siNA) MOLECULES CONTAINING A 2' INTERNUCLEOSIDE LINKAGE
First Claim
1. A short interfering nucleic acid (siNA) molecule capable of mediating RNA interference comprising an antisense strand that is complementary to a nucleic acid target, wherein said antisense strand comprises a 5′
- modified nucleotide having Formula II;
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Abstract
The present invention relates to RNAi molecules, and compositions thereof, comprising a 2′ internucleoside linkage connecting the nucleotide at position 1 and the nucleotide at position 2 at the 5′ end of the antisense strand. Specifically, the invention relates to single- and double-stranded short interfering nucleic acid (siNA) molecules that are capable of mediating RNA interference comprising 5′ modified nucleotides that comprise, among other potential modifications, a 2′ internucleoside linkage. The invention further relates to 5′ modified nucleotides used as reagents to generate the RNAi molecules of the invention and methods of using the disclosed RNAi molecules.
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Citations
46 Claims
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1. A short interfering nucleic acid (siNA) molecule capable of mediating RNA interference comprising an antisense strand that is complementary to a nucleic acid target, wherein said antisense strand comprises a 5′
- modified nucleotide having Formula II;
- View Dependent Claims (2, 3, 4, 7, 8, 9, 10, 13, 15, 16, 17, 18, 23, 24, 30, 31)
- modified nucleotide having Formula II;
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5-6. -6. (canceled)
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11-12. -12. (canceled)
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14. (canceled)
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19-22. -22. (canceled)
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25-29. -29. (canceled)
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32-35. -35. (canceled)
- 36. A modified nucleotide having the structure of Formula I:
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41-42. -42. (canceled)
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44-45. -45. (canceled)
Specification