METHOD OF INACTIVATING A GLUCOCORTICOID RECEPTOR GENE IN AN ISOLATED CELL
First Claim
1. A zinc finger nuclease (ZFN) fusion protein comprising a cleavage domain and a zinc-finger protein (ZFP) that binds to a target site in a glucocorticoid receptor (GR) gene, wherein the ZFP comprises 4 or 5 engineered zinc-finger binding domains, each zinc finger domain comprising a recognition helix region designated F1 to F4 or F1 to F5, wherein the zinc finger protein comprises the recognition helix regions as shown in a single row of Table 2 and further wherein the target site is selected from the group consisting of SEQ ID NOs:
- 1 to 7.
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Accused Products
Abstract
Disclosed herein are methods and compositions for inactivation of the human glucocorticoid receptor (GR) gene by targeted cleavage of genomic DNA encoding the GR. Such methods and compositions are useful, for example, in therapeutic applications which require retention of immune function during glucocorticoid treatment.
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Citations
17 Claims
- 1. A zinc finger nuclease (ZFN) fusion protein comprising a cleavage domain and a zinc-finger protein (ZFP) that binds to a target site in a glucocorticoid receptor (GR) gene, wherein the ZFP comprises 4 or 5 engineered zinc-finger binding domains, each zinc finger domain comprising a recognition helix region designated F1 to F4 or F1 to F5, wherein the zinc finger protein comprises the recognition helix regions as shown in a single row of Table 2 and further wherein the target site is selected from the group consisting of SEQ ID NOs:
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13. The method of claim 13, wherein the corticosteriod is dexamethasone.
Specification