GENERATION OF ACYL AMINO ACIDS
First Claim
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1. A method of making an acyl amino acid composition by contacting an engineered peptide synthetase with an amino acid substrate and an acyl entity substrate for the engineered peptide synthetase, under conditions and for a time sufficient for an acyl amino acid composition to be made.
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Abstract
Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides.
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Citations
21 Claims
- 1. A method of making an acyl amino acid composition by contacting an engineered peptide synthetase with an amino acid substrate and an acyl entity substrate for the engineered peptide synthetase, under conditions and for a time sufficient for an acyl amino acid composition to be made.
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8. A cell engineered to express at least one engineered peptide synthetase that synthesizes an acyl amino acid.
- 9. An acyl amino acid composition produced by an engineered peptide synthetase.
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12. A method of preparing a product comprising:
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providing or obtaining an acyl amino acid composition prepared in an engineered microbial cell; enriching the acyl amino acid composition for a particular acyl amino acid; combining the enriched acyl amino acid composition with at least one other component to produce a product.
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13. A method comprising steps of:
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contacting an engineered peptide synthetase polypeptide that comprises a single peptide synthetase domain and lacks a thioesterase domain, and/or a reductase domain with; an amino acid substrate of the peptide synthetase polypeptide; and an acyl moiety substrate of the peptide synthetase polypeptide, the contacting being performed under conditions and for a time sufficient that the engineered peptide synthetase polypeptide covalently links the acyl moiety from the acyl moiety substrate to the amino acid so that an acyl amino acid is generated. - View Dependent Claims (14, 15, 16, 18)
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Specification