WIREFRAME NANOSTRUCTURES

• US 20160082108A1
• Filed: 09/30/2015
• Published: 03/24/2016
• Est. Priority Date: 04/17/2007
• Status: Abandoned Application

• Current Assignee: Dana-Farber Cancer Institute
• Original Assignee: Dana-Farber Cancer Institute
• Inventors: Shih, William M.
• Application Number:
  US14/870,756
• Publication Number:
  US 20160082108A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 9/0092 Hollow drug-filled fibres, tubes of the core-shell type, coated fibres, coated rods, microtubules or nanotubes
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12N 15/10 Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12Q 1/68 involving nucleic acids

Abstract

The present invention generally relates to nanotechnology and, in particular, to wireframe nanostructures which may be formed from nucleic acids. In various aspects, the invention relates to molecular structures having a plurality of vertices and pathways connecting the vertices, which may be formed from nucleic acids, including bundles or tubes of nucleic acids. Such molecular structures may form shapes such as icosahedrons, octahedrons, tetrahedrons, or other polyhedra, which may define an interior space. The interior space may be used, for example, to contain a molecule for further study, or to contain a molecule for drug delivery purposes. In some cases, the molecular structure may be stabilized using relatively short nucleic acid strands that interact with two or more nucleic acid portions within the structure, thereby substantially immobilizing the portions relative to each other. Other aspects of the invention relate to techniques for forming such molecular structures, techniques for using such molecular structures, techniques of promoting such molecular structures, kits involving such molecular structures, and the like.

Claims

1-118. -118. (canceled)

119. A composition, comprising:

a solution, comprising a plurality of substantially identical molecular structures, each molecular structure defining a plurality of vertices and pathways forming a polyhedral structure defining a hollow three-dimensional interior space, the vertices each having at least three pathways emanating therefrom, each pathway connecting two vertices, wherein each pathway connecting two vertices comprises a nanotube comprising a plurality of nucleic acids, wherein the molecular structure has a smallest dimension in any direction that is at least about 100 nm.

120. The composition of claim 119, wherein the molecular structure is substantially rigid.

121. The composition of claim 119, wherein at least one of the nucleic acids has a length of at least about 500 nucleotides.

122. The composition of claim 119, wherein the nucleic acids forming the nanotubes comprise one or more nucleic acid stabilizers, at least some of which comprise a nucleic acid having a length of less than about 100 nucleotides.

123. The composition of claim 119, wherein the nanotubes each comprise a six-helix nucleic acid bundle.

124. The composition of claim 119, wherein at least one pathway connecting two vertices comprises a ten-helix nucleic acid bundle of nucleic acid.

125. The composition of claim 119, wherein at least one vertex has at least four pathways emanating therefrom.

126. The composition of claim 119, wherein the molecular structure comprises at least four vertices.

127. The composition of claim 119, wherein the plurality of vertices defines faces of the molecular structure, wherein each face of the molecular structure is defined by only three vertices.

128. A composition, comprising:

a solution, comprising a plurality of substantially identical molecular structures, each molecular structure defining a plurality of vertices and pathways forming a polyhedral structure defining a hollow three-dimensional interior space, the vertices each having at least three pathways emanating therefrom, each pathway connecting two vertices, wherein each pathway connecting two vertices comprises nanotubes comprising a plurality of nucleic acids and each pathway has a length between the two vertices of at least about 100 nm.

129. The composition of claim 128, wherein the nucleic acids forming the nanotubes comprise one or more nucleic acid stabilizers at least some of which comprise a nucleic acid having a length of less than about 100 nucleotides.

130. A composition, comprising:

a solution, comprising a plurality of substantially identical molecular structures, each molecular structure defining a polyhedral structure comprising a plurality of vertices and pathways defining a hollow three-dimensional interior space, the vertices each having at least three pathways emanating therefrom, each pathway connecting two vertices, each pathway of the molecular structure being formed from a nanotube comprising a plurality of nucleic acids, and the interior space having a smallest dimension in any direction of at least about 100 nm and being free of the nucleic acids forming the molecular structure.