ELECTROCHEMICAL FLOW-CELL FOR HYDROGEN PRODUCTION AND NICOTINAMIDE DEPENDENT TARGET REDUCTION, AND RELATED METHODS AND SYSTEMS
First Claim
1. A system for hydrogen production, the system comprising a nanolipoprotein particle presenting a nicotinamide co-factor dependent membrane hydrogenase, at least two opposing electrodes, an electrically conductive supporting structure between said first electrode and second electrode, and, wherein the nanolipoprotein particles are immobilized to the electrically conductive supporting structure.
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Abstract
Methods and systems for hydrogen production or production of a reduced target molecule are described, wherein a nicotinamide co-factor dependent membrane hydrogenase or a nicotinamide co-factor dependent membrane enzyme presented on a nanolipoprotein adsorbed onto an electrically conductive supporting structure, which can preferably be chemically inert, is contacted with protons or a target molecule to be reduced and nicotinamide cofactors in presence of an electric current and one or more electrically driven redox mediators. Methods and systems for production of hydrogen or a reduced target molecule are also described wherein a membrane-bound hydrogenase enzyme or enzyme capable or reducing a target molecule is contacted with protons or the target molecule, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production or production of a reduced target molecule in presence of an electrical current and of an electrically driven redox mediator.
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Citations
38 Claims
- 1. A system for hydrogen production, the system comprising a nanolipoprotein particle presenting a nicotinamide co-factor dependent membrane hydrogenase, at least two opposing electrodes, an electrically conductive supporting structure between said first electrode and second electrode, and, wherein the nanolipoprotein particles are immobilized to the electrically conductive supporting structure.
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14. A method to produce hydrogen, the method comprising
combining protons, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle immobilized on an electrically conductive supporting structure for a time and under condition to allow hydrogen production in presence of an electrical current and of an electrically driven redox mediator.
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21. A system for hydrogen production, the system comprising a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle;
- and an electrochemical flow cell comprising a first electrode and a second electrode, an electrically conductive supporting structure wherein the electrochemical flow cell is configured to receive a solution in a space between the first electrode and the second electrode, the electrically conductive supporting structure is configured to immobilize the nicotinamide co-factor dependent membrane hydrogenase presented on the nanolipoprotein particle and to be exposed to the solution in the electrochemical flow cell.
- View Dependent Claims (22, 23, 24, 25, 26)
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27. A method to produce hydrogen, the method comprising:
contacting protons, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production in presence of an electrical current and of an electrically driven redox mediator. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
Specification