IDENTIFYING IONIZABLE SPECIES WITH VOLTAMMETRIC DUTY CYCLES

• US 20160084791A1
• Filed: 12/03/2015
• Published: 03/24/2016
• Est. Priority Date: 07/10/2008
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Bayer Healthcare LLC (Bayer AG)
• Inventors: Wu, Huan-Ping
• Granted Patent: US 9,726,632 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage

Abstract

A sensor system including devices and methods for determining the concentration of an analyte in a sample is described. Input signals including amperometric and voltammetric duty cycles of excitations and relaxations may provide a shorter analysis time and/or improve the accuracy and/or precision of the analysis. The disclosed system may reduce analysis errors, thus improving measurement performance, by adjusting the potential and/or scan rate in response to output currents obtained from voltammetric scans. The disclosed system also may determine the concentration of more than one ionizable species in the sample by adjusting the potential and/or scan rate in response to output currents obtained from voltammetric scans. The multiple, determined concentrations may be used to determine the concentration of multiple analytes or to correct the concentration determined for an analyte, thus improving the measurement performance of the system.

Claims

1-9. -9. (canceled)

10. A method for identifying and/or measuring an ionizable species in a sample, the method comprising:

applying an electric input signal to the sample, the electric input signal including a plurality of duty cycles, each duty cycle of the plurality of duty cycles including;

exciting the sample with an electrical pulse during an excitation period; and

relaxing the sample after the electrical pulse during a relaxation period;

detecting an output signal of the sample responsive to the electric input signal; and

identifying and/or measuring the ionizable species in the sample based on the output signal.

11. The method of claim 10, wherein an electric current of the electric input signal is reduced to at least one-half of an electric current at an excitation maximum during the relaxation periods.

12. The method of claim 10, wherein an electric current of the electric input signal is reduced by at least an order of magnitude relative to an electric current at an excitation maximum during the relaxation periods.

13. The method of claim 10, wherein an electric current to the sample is reduced to a zero electric current state during the relaxation periods.

14. The method of claim 13, wherein the electric input signal is applied to the sample through an electric circuit including at least one working electrode and at least one counter electrode at a sensor strip containing the sample, the method further comprising:

opening the electric circuit to provide the zero electric current state.

15. The method of claim 10, wherein the plurality of duty cycles of the electric input signal includes at least three duty cycles.

16. The method of claim 10, wherein the plurality of duty cycles of the electric input signal is applied within about 30 seconds.

17. The method of claim 10, wherein the plurality of duty cycles of the electric input signal includes at least four duty cycles applied within about seven seconds.

18. The method of claim 10, wherein each excitation period of the plurality of duty cycles is about 0.3 to 0.8 seconds long.

19. The method of claim 10, wherein each duty cycle of the plurality of duty cycles has a length of less than three seconds.

20. The method of claim 10, wherein each excitation period of the plurality of duty cycles is about 0.3 to 0.5 seconds long, and each duty cycle of the plurality of duty cycles has an interval of less than two seconds.

21. The method of claim 10, further comprising:

adjusting the electric input signal based on the output signal responsive to at least one duty cycle, the at least one duty cycle including a voltammetric pulse.

22. The method of claim 10, wherein at least one electrical pulse of the electrical pulses is a linear voltammetric pulse, a cyclic voltammetric pulse, or an acyclic voltammetric pulse.

23. The method of claim 22, wherein the at least one electrical pulse is an acyclic voltammetric pulse that includes a larger forward or reverse current peak than the other of the forward or reverse current peak.

24. The method of claim 22, wherein the at least one electrical pulse is an acyclic voltammetric pulse that starts and ends at substantially a same voltage when started at most 20 millivolts away from a formal potential E^0′

of a redox couple of the sample.

25. The method of claim 22, wherein the at least one electrical pulse is an acyclic voltammetric pulse that includes a forward and a reverse increase in voltage that substantially exclude oxidation and reduction output current peaks of a redox couple of the sample.

26. The method of claim 22, wherein the at least one electrical pulse is a linear voltammetric pulse that includes an increase in voltage at a fixed rate.

27. The method of claim 22, wherein the at least one electrical pulse is a cyclic voltammetric pulse that includes an increase and a decrease in voltage at a fixed rate and includes oxidation and reduction peaks of a redox couple of the sample.

28. The method of claim 22, wherein a preceding or a following electrical pulse to the at least one electrical pulse is an amperometric pulse.

29. The method of claim 10, wherein the identifying and/or measuring of the ionizable species in the sample is based on the output signal responsive to the excitation periods.