NOVEL ANTIBODIES
First Claim
Patent Images
1. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3ε
- , wherein said epitope comprises amino acid residue N4 as residue that is critical for binding.
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Accused Products
Abstract
The present invention relates to novel antibodies, which combine high affinity with high potency, particularly novel antibodies against a novel epitope.
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Citations
26 Claims
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1. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3ε
- , wherein said epitope comprises amino acid residue N4 as residue that is critical for binding.
- View Dependent Claims (3, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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2. The isolated antibody or functional fragment thereof of claim 2, wherein said epitope further comprises amino acid residue E6 as residue that is involved in binding.
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4. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3, wherein said antibody or functional fragment thereof, is binding to human CD3 with a dissociation constant for monovalent binding of less than 3.0×
- 10−
8 M, particularly less than 1.5×
10−
8 M, more particularly less than 1.2×
10−
8 M, and most particularly less than 1.0×
10−
8 M. - View Dependent Claims (9, 14)
- 10−
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5. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3, wherein said antibody or functional fragment thereof, when tested in an IgG format, upon cross-linking, is inducing T-cell activation at least 1.5-fold stronger than antibodies OKT-3 or TR66 after 24 h of stimulation at an IgG concentration of 1.25 μ
- g/ml.
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6. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3, wherein said antibody or functional fragment thereof, when tested in an IgG format, upon cross-linking, is resulting in T-cell activation, which lasts longer than with antibodies OKT-3 or TR66 as indicated by at least 1.5-fold greater increase in CD69 expression after 72 hours of stimulation at an IgG concentration of 1.25 μ
- g/ml.
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7. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3, wherein said antibody or functional fragment thereof, when tested in an IgG format, upon cross-linking, is resulting in a dose-dependent homogeneous activation state of T-cells.
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8. An isolated antibody or functional fragment thereof comprising an antigen-binding region that is specific for an epitope of human CD3, wherein said antibody or functional fragment thereof, when tested in an IgG format, (i) is binding to human CD3 with a dissociation constant for monovalent binding of less than 3.0×
- 10−
8 M, particularly less than 1.5×
10−
8 M, more particularly less than 1.2×
10−
8 M, and most particularly less than 1.0×
10−
8 M; and
(iia), upon cross-linking, is inducing T-cell activation at least 1.5-fold stronger than antibodies OKT-3 or TR66 after 24 h of stimulation at an IgG concentration of 1.25 μ
g/ml;
(iib) is resulting in T-cell activation, which lasts longer than with antibodies OKT-3 or TR66 as indicated by at least 1.5-fold greater increase in CD69 expression after 72 hours of stimulation at an IgG concentration of 1.25 μ
g/ml;
(iic) is resulting in a dose-dependent homogeneous activation state of T-cells; and
/or (iid) is specific for an epitope of human CD3ε
, wherein said epitope comprises amino acid residue N4 as residue that is critical for binding.
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Specification