COMPOSITIONS CONTAINING IDENTIFIER SEQUENCES ON SOLID SUPPORTS FOR NUCLEIC ACID SEQUENCE ANALYSIS
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Abstract
Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
13 Citations
21 Claims
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1. (canceled)
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2. A collection of solid supports comprising single-stranded solid phase nucleic acid species, wherein:
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(a) the solid phase nucleic acid species on each solid support in the collection comprise an identifier sequence and a probe sequence; (b) at least one nucleic acid of the solid phase nucleic acid species of each of the solid supports in the collection has a unique probe sequence different than a probe sequence of the solid phase nucleic acid species of the other solid supports in the collection; (c) the probe sequence is complementary to a nucleotide sequence in a sample nucleic acid; (c) the solid phase nucleic acid species of the solid support share a common probe sequence or do not share a common probe sequence; and (d) the solid phase nucleic acid species of each of the solid supports in the collection share a unique identifier sequence different than the identifier sequences of the solid phase nucleic acid species of the other solid supports in the collection. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21)
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20. A substrate comprising a collection of solid supports that are beads, wherein the beads are oriented in an array and wherein:
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(a) at least one solid phase nucleic acid species of each of the beads in the collection has a unique probe sequence different than a probe sequence of the solid phase nucleic acid species of the other beads in the collection; and (b) the solid phase nucleic acid species of each bead in the collection share a unique identifier sequence different than the identifier sequences of the solid phase nucleic acid species of the other beads in the collection.
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Specification