IMAGE FORMING CYTOMETER

• US 20160103058A1
• Filed: 05/28/2014
• Published: 04/14/2016
• Est. Priority Date: 05/28/2013
• Status: Active Grant

• Current Assignee: ChemoMetec A/S
• Original Assignee: ChemoMetec A/S
• Inventors: Glensbjerg, Martin, Kjaerulff, Sren, Holm, Johan, Ravn Hansen, Frans Ejner
• Granted Patent: US 10,458,896 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/0227 using imaging; using holography
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1475 using image analysis for extracting features of the particle
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1438 Using two lasers in succession
• CPC Class Codes: G01N 2015/144 Imaging characterised by its optical setup
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G01N 2015/1495 Deformation of particles
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/10064 Fluorescence image
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts

Abstract

The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field, dark field or one or more sources of excitation light. The system comprises illumination means (11, 112), a sample holder (100), a sample compartment (101), imaging means (120), collection means (121), light modulation means (122, 123), and detection means (130) with active detection elements (131).

Claims

1. An image cytometer, comprising:

a first light source configured for emitting light into a sample region,

focusing means for forming collimated light and directing the collimated light from the first light source along an optical axis of the cytometer;

a second light source comprising a first excitation light source configured for emitting excitation light into the sample region;

image forming means for forming an image of at least part of the sample region on an array of detection elements, wherein

the sample region is located between the focusing means and the array of detection elements, and wherein

the cytometer is able to be configured to be interchanged between a bright field mode, a dark field mode and a fluorescence mode, and/or wherein

the first light source is configured for emitting light with a wavelength less than 400 nm, and/or wherein

the excitation light is at an incidence angle relative to the optical axis so as to provide the fluorescence mode.

2-123. -123. (canceled)