PROCESS AND METHOD FOR IMPROVING FERMENTATION BY THE ADDITION OF HYDROTHERMALLY TREATED STILLAGE

• US 20160115503A1
• Filed: 05/27/2014
• Published: 04/28/2016
• Est. Priority Date: 05/24/2013
• Status: Abandoned Application

• Current Assignee: Valicor, Inc.
• Original Assignee: Valicor, Inc.
• Inventors: Bleyer, James R., Czartoski, Thomas
• Application Number:
  US14/893,577
• Publication Number:
  US 20160115503A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12N 1/005 after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A method of improving fermentation by increasing the bioavailability of components in stillage, using all or a portion of the hydrothermally treated stillage as a component of a media, and using the media for a process such as fermentation or biomass production. The metabolites and biomass recovered from the method above. Media including hydrothermally treating stillage obtained by heating the stillage to a temperature of 190° F. to 300° F. A method of improving fermentation by removing solids in stillage, hydrothermally treating the stillage by heating the stillage to a temperature of 190° F. to 300° F., using all or a portion of the stillage as a component of a media, and using the media for a process chosen from the group consisting of fermentation and biomass production.

Claims

1. A method of improving fermentation, including the steps of:

increasing the bioavailability of components in stillage;

using all or a portion of the stillage as a component of a media; and

using the media for a process chosen from the group consisting of fermentation and biomass production.

2. The method of claim 1, wherein said increasing bioavailability step is further defined as a step chosen from the group consisting of hydrolyzing oligosaccharides into monosaccharides and disaccharides, unfolding protein matrixes, denaturing protein, hydrolyzing protein, and combinations thereof.

3. The method of claim 1, wherein said increasing the bioavailability further includes the step of hydrothermally treating the stillage by heating the stillage to a temperature of 190°

F. to 300°

F.

4. The method of claim 3, wherein said hydrothermally treating step is further defined as holding the stillage at the treatment temperature for 3 to 180 minutes and at a pressure at or above the saturation pressure of the stillage.

5. The method of claim 1, wherein the fermentation produces a metabolite chosen from the group consisting of organic acids, alcohols, lipids, carbohydrates, proteins, and secondary metabolites.

6. The method of claim 1, wherein the biomass is chosen from the group consisting of algae, bacteria, yeast, fungi, archae, and cultured cells.

7. The method of claim 3, wherein organic compounds in the hydrothermally treated stillage provide all or a portion of the carbon source in the media.

8. The method of claim 3, wherein the hydrothermally treated stillage provides all or a portion of the nutrient requirements.

9. The method of claim 1, further including the step of adding a composition to the media from the group consisting of a carbon source and nutrients.

10. The method of claim 9, wherein the carbon source is chosen from the group consisting of dextrose, sucrose, fructose, xylose, arabinose, other carbohydrates, organic acids, glycerol, ethanol, other alcohols, carbon monoxide, carbon dioxide, methane, and a carbon source derived from cellulosic material.

11. The method of claim 3, wherein the hydrothermally treated stillage makes up all or a portion of the media.

12. The method of claim 1, wherein the stillage is chosen from the group consisting of thin stillage, whole stillage, and thick stillage.

13. The method of claim 12, wherein the stillage is thick stillage and is produced by a method chosen from the group consisting of:

removal of water from thin stillage to concentrate solids, filtration of thin stillage to concentrate solids, centrifugation of whole stillage under centrifuge operating conditions that promote transport of more solids into the centrate and less into the wet cake fraction, addition of solids to thin stillage, particle size reduction of stillage to allow for additional fine solids to be transported into the centrate fraction during separation of stillage, particle size reduction of the corn slurry prior to fermentation to allow for additional fine solids to be transported into the stillage fraction during separation, and combinations thereof.

14. The method of claim 1, further including the step of performing a particle size reduction process on all or a portion of the stillage prior to or after said hydrothermally treating step, wherein the stillage is chosen from the group consisting of thin stillage, whole stillage, wet cake, and thick stillage.

15. The method of claim 5, further including the steps of separating and recovering metabolites and recovering biomass from the fermentation media.

16. The method of claim 1, further including the step of using the fermentation effluent in an additional alcohol fermentation process.

17. The method of claim 3, further including the steps of producing stickwater by removing suspended solids from the hydrothermally treated stillage by a method chosen from the group consisting of centrifuges, decanting centrifuges, filter centrifuge, filters, membranes, hydrocyclone, quiescent decantation, dissolved air floatation, and flocculation;

and adding all or a portion of the stickwater to an ethanol fermentation step or an operation upstream of fermentation.

18. The metabolites recovered from the method of claim 5.

19. The biomass recovered from the method of claim 6.

20. Media including hydrothermally treating stillage obtained by heating the stillage to a temperature of 190°

F. to 300°

F.

21. A method of improving fermentation, including the steps of:

removing solids in stillage;

hydrothermally treating the stillage by heating the stillage to a temperature of 190°

F. to 300°

F.;

using all or a portion of the stillage as a component of a media; and

using the media for a process chosen from the group consisting of fermentation and biomass production.

22. The method of claim 21, wherein said removing solids step is further defined as removing solids chosen from the group consisting of suspended solids, dissolved solids, oil, proteins, fiber, and ash.

23. The method of claim 21, wherein said removing step is performed by a mechanism chosen from the group consisting of centrifuges, decanting centrifuges, filter centrifuge, filters, membranes, hydrocyclone, quiescent decantation, dissolved air floatation, flocculation, biological remediation, electro-dialysis, ion exchange, distillation, solvent extraction, and precipitation.

24. The method of claim 21, further including the step of adding one or more agents to assist in the removal of solids chosen from the group consisting of acids, bases, minerals, polymeric flocculants, microparticulate settling aids, precipitation aids, and salts.

25. The method of claim 21, further including the step of adjusting the temperature to assist in the removal of solids.