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Compositions, Methods and Apparatus for Oligonucleotides Synthesis

  • US 20160122755A1
  • Filed: 03/13/2014
  • Published: 05/05/2016
  • Est. Priority Date: 03/13/2013
  • Status: Active Grant
First Claim
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1. A method for generating an oligonucleotide having a predefined sequence, the method comprising:

  • a. designing at least one plurality of oligonucleotides, each oligonucleotide of each plurality of oligonucleotides having a different length, each oligonucleotide having an identical predefined internal sequence, a 3′

    flanking sequence at the 3′

    end of the internal sequence and a 5′

    flanking sequence at the 5′

    end of the internal sequence, the 3′

    flanking sequence comprising a primer recognition site and a restriction enzyme recognition site and the 5′

    flanking sequence comprising a primer recognition site, a restriction enzyme recognition site and an additional padding nucleotide sequence, wherein each padding nucleotide sequence has a different length;

    b. synthesizing the at least one plurality of oligonucleotides on a solid support; and

    c. isolating the oligonucleotide having the correct predefined internal sequence.

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