SIGNAL AMPLIFICATION OF FLUORESCENCE IN SITU HYBRIDIZATION
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Abstract
Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.
36 Citations
39 Claims
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1-20. -20. (canceled)
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21. A method of detecting a target nucleic acid, the method comprising:
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(a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe is double-stranded and comprises a capture tag in only one strand of the probe; (b) hybridizing the probe with the target nucleic to produce a duplex comprising the capture tag; (c) linking the probe in the duplex to a signal amplification conjugate via the capture tag; and (d) producing an amplified detectable signal from the signal amplification conjugate in the duplex. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification