MICROPLATES FOR MAGNETIC 3D CULTURE

• US 20160137974A1
• Filed: 12/10/2015
• Published: 05/19/2016
• Est. Priority Date: 09/25/2008
• Status: Abandoned Application

• Current Assignee: Greiner Bio-One North America Inc. (Greiner AG)
• Original Assignee: Nano3D Biosciences, Inc.
• Inventors: SOUZA, Glauco
• Application Number:
  US14/965,702
• Publication Number:
  US 20160137974A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/10 Petri dish crystallising dishes B01L3/06
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 33/00 Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02
• CPC Class Codes: C12M 35/06 Magnetic means C12M35/02 takes precedence
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture

Abstract

Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same.

Claims

1. A magnetic culture plate, comprising

a) a cell culture vessel and a cap for said vessel,

b) said cap having a lip around an outer circumference thereof and being shaped to fit over or under said cell culture vessel,

c) said cap having a depression into which a magnet is affixed, thus holding said magnet over said culture vessel when said cap is in place over said vessel.

2. The magnetic culture plate of claim 1, wherein said vessel has a plurality of wells, and said cap has a plurality of depressions, in each of which a magnet is affixed, such that every magnet is orientated in an opposite orientation to an adjacent magnet.

3. The magnetic culture plate of claim 1 or 2, said magnet being adhered to a bottom of said depression.

4. The magnetic culture plate of claim 1 or 2, said magnet being snap fit into said depression.

5. A multiwell cell culture vessel, comprising a vessel having plurality of wells in an array, a cap covering vessel and said plurality of wells, said cap having a plurality of magnets in an array, each magnet affixed to a depression in said cap or on a post on said cap, such that each of said wells has a magnet over said well when said cap is in place over said vessel.

6. The multiwell magnetic cell culture vessel of claim 6, each adjacent magnet being affixed in opposite polarity.

7. A magnetic cap for a microplate, comprising a cap sized to cover a standard ANSI-SLAS microplate having a plurality of wells in an array, said cap having lip around a circumference thereof and a plurality of magnets in an array, each magnet affixed to a depression in said cap or on a post on said cap, such that each magnet sits over a well when said cap is in place over or under said microplate and such that each adjacent each adjacent magnet is affixed in opposite polarity.

8. The magnetic cap of claim 8, having 6 magnets of 20-100 lbs pull force, 10000-15000 Gauss Br_max and 30-60 MGOe Bh_max.

9. The magnetic cap of claim 8, having 12 magnets of 2-20 lbs pull force, 10000-15000 Gauss Br_max and 30-60 MGOe Bh_max.

10. The magnetic cap of claim 8, having 24 magnets of 2-10 lbs pull force, 10000-15000 Gauss Br_max and 30-60 MGOe Bh_max.

11. The magnetic cap of claim 8, having 96 magnets of 0.5-2 lbs pull force, 10000-15000 Gauss Br_max and 30-60 MGOe Bh_max.

12. The magnetic cap of claim 8, having 384 magnets of 0.05-1 lbs pull force, 10000-15000 Gauss Br_max and 30-60 MGOe Bh_max.

13. The magnetic cap of claim 8, having the dimensions of FIG. 7, 8, 9, 10, 11 or 12.

14. The magnetic cap of claim 8, said magnets having the dimensions and strengths of FIG. 13.

19. A method of 3D cell culture, comprising:

a) incubating one or more cell types in a solution of iron oxide nanoparticles until said cell types contain about 30-150 pg/cell of iron oxide;

b) suspending said cell types containing said iron oxide in a culture medium;

c) aliquoting samples of said suspended cell types to one or more wells of a microplate;

d) placing the magnetic cap of claim 8 above or below said microplate;

e) incubating said microplate until a 3D culture of cells or desired 3D culture printed shape is formed; and

f) incubating said microplate under the influence of the magnetic field for the duration of the 3D culture.

15. A method of making a magnetic driver for a standard microtiter plate, said method comprising:

a) obtaining a cap having a circumferential lip that fits both over and under a separate microtiter plate having a plurality of wells; and

b) affixing a plurality of magnets to said cap such that every well has a magnet over said well when said cap is in place over or under said microtiter plate, and such that every magnet has a polarity opposite each adjacent magnet.

16. The method of claim 16, wherein said affixing step is snap-fitting each magnet to a depression in an upper surface of said cap.

17. The method of claim 16, wherein said affixing step is adhering each magnet to a depression in an upper surface of said cap.

18. The method of claim 16, wherein said affixing step is fitting a ring magnet over a post on a surface of said cap.