GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM NORMAL HUMAN MAMMARY EPITHELIAL CELLS
First Claim
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1. A method of generating human mammary-derived induced pluripotent stem cells (m-iPSCs), comprising:
- providing a quantity of human mammary cells;
transfecting the human mammary cells with at least one vector encoding at least one reprogramming factor;
plating the cells on a culture vessel coated with a substrate; and
culturing the cells in an induction media, wherein the transfection, plating and culturing generates colonies of human mammary-derived induced pluripotent stem cells (m-iPSCs).
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Abstract
Described herein are reprogramming techniques allowing for production of mammary-derived iPSCs (“m-iPSCs”). The m-iPSCs described herein exhibit all the hallmarks of stem cell identity including round cluster, bright colony morphology, clonal expansion, and pluripotent marker expression (alkaline phosphatase expression, Oct-4, nanog, etc.) Further refined techniques allow for generation of m-iPSCs under essentially defined conditions.
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Citations
22 Claims
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1. A method of generating human mammary-derived induced pluripotent stem cells (m-iPSCs), comprising:
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providing a quantity of human mammary cells; transfecting the human mammary cells with at least one vector encoding at least one reprogramming factor; plating the cells on a culture vessel coated with a substrate; and culturing the cells in an induction media, wherein the transfection, plating and culturing generates colonies of human mammary-derived induced pluripotent stem cells (m-iPSCs). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of reprogramming a human mammary cell, comprising:
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providing a quantity of human mammary cells; culturing the human mammary cells in the presence of at least one vector and/or at least one reprogramming agent; plating the cells on a culture vessel coated with a substrate; and culturing the cells in an induction media, wherein the transfection, plating and culturing reprograms the human mammary cells to a less differentiated state. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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Specification