Methods of Use of Seed-Origin Endophyte Populations
First Claim
1. A synthetic combination comprising a purified bacterial population in association with a seed of a cereal agricultural plant, wherein the purified bacterial population comprises a seed bacterial endophyte that is heterologous to the seed, has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 521, 533, 560, 588, 658, 710, 856 and 1447, and is capable of producing an auxin or acetoin, and wherein the seed bacterial endophyte is present in the synthetic combination at a concentration of at least 103 CFU/seed on the surface of a seed and in an amount effective to increase tolerance to drought of a cereal agricultural plant produced from the seed as compared to a reference plant grown under the same conditions.
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Abstract
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.
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Citations
20 Claims
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1. A synthetic combination comprising a purified bacterial population in association with a seed of a cereal agricultural plant, wherein the purified bacterial population comprises a seed bacterial endophyte that is heterologous to the seed, has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 521, 533, 560, 588, 658, 710, 856 and 1447, and is capable of producing an auxin or acetoin, and wherein the seed bacterial endophyte is present in the synthetic combination at a concentration of at least 103 CFU/seed on the surface of a seed and in an amount effective to increase tolerance to drought of a cereal agricultural plant produced from the seed as compared to a reference plant grown under the same conditions.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A synthetic combination comprising a purified bacterial population in association with a seed of a cereal agricultural plant, wherein the purified bacterial population comprises a first bacterial endophyte and a second bacterial endophyte, wherein the first bacterial endophyte and second bacterial endophyte has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 521, 533, 560, 588, 658, 710, 856 and 1447, and is capable of producing an auxin or acetoin, wherein the first bacterial endophyte and the second bacterial endophyte have different 16S nucleic acid sequence, and wherein the first bacterial endophyte and second bacterial endophyte are present in the synthetic combination at a concentration of at least 103 CFU/seed on the surface of a seed and in an amount effective to increase tolerance to drought of a cereal agricultural plant produced from the seed as compared to a reference plant grown under the same conditions.
- View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
Specification