Single-Walled Carbon Nanotube Biosensor for Detection of Glucose, Lactate, and Urea
First Claim
1. A microscale biosensor for detecting a chemical agent, the biosensor comprising:
- a substrate;
a conductive layer attached to a surface of the substrate, the conductive layer forming at least one pair of electrodes with an insulating gap between the electrodes; and
a conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the electrodes and bridging the gap between the electrodes;
wherein the one or more nanotubes are functionalized via a linker with an enzyme that reacts with said chemical agent, whereby the conductivity of the conductive bridge is modified; and
wherein the linker is 1-pyrenebutanoic acid succinimidyl ester.
2 Assignments
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Accused Products
Abstract
A single-walled carbon nanotube-based micron scale multiplex biosensor is provided that enables the detection of glucose, lactate, and urea. The sensor is based on modification of semiconducting single-walled carbon nanotubes using a linker that non-covalently associates with the nanotubes and covalently couples to an enzyme. Reaction of a physiological substrate with the enzyme results in increased resistance of the nanotubes within the sensor. The sensor is suitable for use in patient monitoring, particularly in a clinical setting. Incorporation of read out electronics and an RF signal generator into the sensor device enables it to communicate to a relay station or remote receiver. Methods are also provided for fabricating the biosensor device and using the device for detection.
8 Citations
27 Claims
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1. A microscale biosensor for detecting a chemical agent, the biosensor comprising:
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a substrate; a conductive layer attached to a surface of the substrate, the conductive layer forming at least one pair of electrodes with an insulating gap between the electrodes; and a conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the electrodes and bridging the gap between the electrodes;
wherein the one or more nanotubes are functionalized via a linker with an enzyme that reacts with said chemical agent, whereby the conductivity of the conductive bridge is modified; and
wherein the linker is 1-pyrenebutanoic acid succinimidyl ester. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 26)
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16. A method of fabricating a biosensor, the method comprising the steps of:
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(a) providing a substrate comprising a nanoscale trench; (b) depositing by fluidic assembly a conductive bridge consisting essentially of one or more single-walled carbon nanotubes into the nanoscale trench, the bridge having first and second ends; (c) depositing first and second conductive electrodes onto the substrate, whereby the first electrode covers and contacts the first bridge end and the second electrode covers and contacts the second bridge end; (d) associating 1-pyrenebutanoic acid succinimidyl ester as a linker with said single-walled carbon nanotubes and removing from the nanotubes any non-associated linker molecules; (e) reacting an enzyme with said nanotube-associated linker; and (f) blocking unreacted nanotube-associated linker using a reagent comprising free amino groups, to obtain said biosensor.
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17-19. -19. (canceled)
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20. A method of fabricating a biosensor, the method comprising the steps of:
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(a) providing a substrate comprising a nanoscale trench; (b) depositing first and second conductive electrodes onto the substrate, the electrodes separated by an insulating gap; (c) depositing by dielectrophoretic assembly a conductive bridge consisting essentially of one or more single-walled carbon nanotubes, the bridge having a first end contacting the first conductive electrode and a second end contacting the second conductive electrode; (d) associating 1-pyrenebutanoic acid succinimidyl ester as a linker with said single-walled carbon nanotubes and removing from the nanotubes any non-associated linker molecules; (e) reacting an enzyme with said nanotube-associated linker; and (f) blocking unreacted nanotube-associated linker using a reagent comprising free amino groups, to obtain said biosensor.
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21-25. -25. (canceled)
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27-35. -35. (canceled)
Specification