Systems, Processes, Methods and Machines for Transforming Image Data Into Sizing and Volume Measurements for Tissue

• US 20160180522A1
• Filed: 02/26/2016
• Published: 06/23/2016
• Est. Priority Date: 10/30/2012
• Status: Active Grant

• Current Assignee: Prodo Laboratories
• Original Assignee: Prodo Laboratories
• Inventors: McCord, Roy
• Granted Patent: US 9,858,664 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G02B 21/008 Details of detection or image processing, including general computer control
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06T 7/60 Analysis of geometric attributes
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts

Abstract

Automated islet measurement systems (AIMS) in combination with tissue volume analysis (TVA) software effectively gauges volumetric and size-based data to generate heretofore unavailable information regarding, for example, populations of islet cells, stem cells and related desiderata.

Claims

1-9. -9. (canceled)

10. An automatic tissue scanning camera (ATSC) system, which comprises, in combination:

a non-phase contrast microscope;

a monochrome charge-coupled device (CCD) camera;

a horizontally-mounted culture flask;

a trans-illumination means, which exceeds the deep field image size by a factor of at least two; and

an automated islet measurement system (AIMS), comprising tissue volume analysis (TVA) software, the AIMS configured to;

identify a cell in the culture flask;

image the cell with the camera, the image comprising pixels;

evaluate a cross-section size of the cell;

measure an optical transmission at each pixel within the cell;

calculate a volume of tissue at each pixel; and

calculate a total volume for the cell.