Microscopy Blade System And Method Of Control

• US 20160187636A1
• Filed: 06/26/2014
• Published: 06/30/2016
• Est. Priority Date: 06/26/2013
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Thompson, Guy, Hinojosa, Christopher D., Fernandez-Alcon, Jose, Ingber, Donald E., Levner, Daniel
• Application Number:
  US14/899,550
• Publication Number:
  US 20160187636A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: G02B 21/02 Objectives
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/14 affording illumination for phase-contrast observation
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/24 Base structure
• CPC Class Codes: G02B 21/26 Stages; Adjusting means therefor
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: G02B 21/36 arranged for photographic purposes or projection purposes G02B21/18 takes precedenceor digital imaging or video purposes including associated control and data processing arrangements image data processing per se G06T
• CPC Class Codes: G02B 7/04 with mechanism for focusing or varying magnification

Abstract

A microscopy system for monitoring of one or more specimens includes a plurality of microscope blades, each microscope blade having at least one objective, at least one illuminator, and at least one detector. The microscopy system also includes a plurality of carriages, each carriage being connected to one or more of the microscope blades, and one or more actuators configured to drive the plurality of carriages along one or more axes, at least some of the plurality of carriages having at least partially overlapping ranges of motion along at least one of the one or more axes. The microscopy system also includes a master controller configured to drive each of the carriages, using the actuator(s), along the one or more axes.

Claims

1. A microscopy system configured for monitoring of one or more specimens, the microscopy system comprising:

a plurality of microscope blades, each of the plurality of microscope blades comprising at least one objective, at least one illuminator, and at least one detector;

a plurality of carriages, each of the plurality of carriages being connected to one or more of the plurality of microscope blades;

one or more actuators configured to drive the plurality of carriages along one or more axes, at least some of the plurality of carriages having at least partially overlapping ranges of motion along at least one of the one or more axes; and

a master controller configured to drive each of the plurality of carriages, using the one or more actuators, along the one or more axes.

2. The microscopy system according to claim 1, further comprising:

a collision avoidance controller configured to control movement of the plurality of microscope blades so that no moving microscope blade contacts any other microscope blade.

3. The microscopy system according to claim 2, wherein the collision avoidance controller is external to the master controller.

4. The microscopy system according to claim 2, wherein the master controller comprises the collision avoidance controller.

5. The microscopy system according to claim 3, wherein the master controller is configured to output mechanical motion requests for each of the plurality of carriages to the one or more actuators to direct movement of each of the plurality of carriages along the one or more axes, at least some of the mechanical motion requests being first passed by the master controller to the collision avoidance controller configured to determine if the at least some of the mechanical motion requests include any mechanical motion requests that would cause any of the plurality of microscope blades to contact any other one of the plurality of microscope blades and to output to the one or more actuators either the at least some of the mechanical motion requests or a corrected set of mechanical motion requests comprising one or more corrected mechanical motion requests together with the subset of the at least some of the mechanical motion requests that were not corrected.

6. The microscopy system according to claim 1, wherein at least one of the plurality of carriages comprises a docking interface bearing one or more mechanical connectors configured to matingly and removably engage corresponding mechanical connectors on the corresponding one of the plurality of microscope blades.

7. The microscopy system according to claim 6, wherein the docking interface further comprises one or more electrical connectors configured to matingly and removably engage one or more corresponding electrical connectors on the corresponding one of the plurality of microscope blades.

8. The microscopy system according to claim 6, wherein the plurality of carriages are independently driven, by the one or more actuators, along parallel axes.

9. The microscopy system according to claim 6, wherein the plurality of carriages are independently driven, by the one or more actuators, along a common axis.

10. The microscopy system according to claim 1, further comprising:

a carriage connected to a plurality of the microscope blades,

wherein the one or more actuators comprise one or more actuators configured to move the carriage bearing the plurality of the microscope blades.

11. The microscopy system according to claim 1, wherein the one or more actuators are configured to move at least one carriage about an axis of rotation.

12. The microscopy system according to claim 1, wherein each of the plurality of microscope blades comprises a focus control system, the focus control system comprising one or more of an electromagnetic motor, piezoelectric motor, sonic motor, voicecoil, or combination thereof.

13. The microscopy system according to claim 6,

wherein at least a plurality of the carriages are ganged together for simultaneous movement along the at least one axis of the one or more axes.

14. The microscopy system according to claim 1, further comprising:

at least one rail along which at least one of the plurality of carriages is disposed to translate.

15. The microscopy system according to claim 14,

wherein the at least one rail comprises a magnetic linear motor rail, and

wherein the at least one of the plurality of carriages, in combination with the magnetic linear motor rail, is configured to levitate with respect to surfaces of the magnetic linear motor rail.

16. The microscopy system according to claim 1,

wherein the one or more actuators configured to drive the plurality of carriages along one or more axes comprises a ball screw, belt, rack, or hydraulic actuator, and

wherein the plurality of carriages driven by the one or more actuators are configured with one or more components adapted to engage the one or more actuators and transmit forces from the one or more actuators to the plurality of carriages.

17. The microscopy system according to claim 1, wherein the one or more specimens comprise a plurality of Organ Chips each having a membrane with cells located thereon, the plurality of microscopy blades for imaging the cells in the plurality of Organ Chips.

18. The microscopy system according to claim 17, wherein each of the plurality of Organ Chips is disposed in a respective Organ Cartridge, each of the plurality of microscope blades being associated with a respective Organ Cartridge.

19. The microscopy system according to claim 18, further comprising:

one or more actuators configured to move the one or more Organ Chips, individually or in combination with one or more of the Organ Cartridge relative to the plurality of microscope blades.

20. The microscopy system according to claim 1, further comprising:

one or more motorized platforms disposed internally to at least one microscope blade of the plurality of microscope blades to move at least one component of an optical train relative to the at least one microscope blade.

21. The microscopy system according to claim 1, wherein the at least one detector comprises an imaging device.

22. The microscopy system according to claim 21, wherein the imaging device comprises a camera.

23. The microscopy system according to claim 21, wherein the at least one illuminator comprises at least one of a metal-halide lamp, a mercury arc-discharge lamp, a xenon lamp, a tungsten-halogen lamp, an incandescent tungsten lamp, a halogen lamp, an arc lamp, a laser, a monochromator, LEDs, OLEDs, or a flash tube.

24. The microscopy system according to claim 21, wherein each of the plurality of microscope blades are configured to support one or more microscopy modalities selected from the group comprising brightfield, darkfield, phase-contrast, epifluorescence, fluorescence, microfluorimetry, confocal, and multi-proton excitation microscopy.

25. The microscopy system according to claim 21, wherein at least one of the plurality of microscope blades comprises a phase condenser.

26. The microscopy system according to claim 24,

wherein a first microscope blade of the plurality of microscope blades is configured to support fluorescence and phase-contrast microscopy modalities, and

wherein a second microscope blade of the plurality of microscope blades is configured to support a confocal microscopy modality.

27. The microscopy system according to claim 1, wherein the plurality of microscope blades are configured to operate in parallel, in series, or a combination thereof.

28. A method of controlling a microscopy system comprising a plurality of movable microscope blades movably disposed along a range of positions along one or more axes, at least some of the plurality of positions for the plurality of movable microscope blades along the range of positions being at least partially overlapping, the method comprising:

using a controller, determining a mechanical motion request for a movable microscope blade disposed at a first location to move to a second location, at least one of the first location or the second location being within the at least partially overlapping ranges of motion along the one or more axes; and

using the controller, or another controller, to cause at least one actuator to move the movable microscope blade from the first location to the second location.

29. The method of controlling the microscopy system according to claim 28, further comprising:

using the controller or the another controller to determine if a correction to the mechanical motion request is required to avoid contact between the movable microscope blade and any of the remainder of the plurality of microscope blades arising from movement of the movable microscope blade in accord with the mechanical motion request and, if so, to output to the movable microscope blade or the at least one actuator a modified mechanical motion request; and

using the controller, or another controller, to cause the at least one actuator to move the movable microscope blade from the first location to the second location in accord with one of the mechanical motion request or the modified mechanical motion request.

30. The method of controlling the microscopy system according to claim 29, wherein a plurality of microscope blades are attached to a plurality of carriages disposed to translate along at least one rail.

31. The method of controlling the microscopy system according to claim 29, wherein the controller comprises a master controller, and

wherein the another controller comprises a collision avoidance controller.

32. The method of controlling the microscopy system according to claim 31, wherein the collision avoidance controller comprises the master controller.

33. The method of controlling the microscopy system according to claim 31, wherein the collision avoidance controller is external to the master controller.

34. The method of controlling the microscopy system according to claim 31, wherein the master controller, singly or in combination with the collision avoidance controller, is configured to cause a plurality of microscope blades to move simultaneously or sequentially along the rail.

35. The method of controlling the microscopy system according to claim 31, wherein the collision avoidance controller is utilized to analyze mechanical motion requests of microscope blades operating within the at least partially overlapping ranges of motion along the one or more axes.