METHOD FOR DETECTING PROSTATIC BASAL CELLS

• US 20160195535A1
• Filed: 12/18/2013
• Published: 07/07/2016
• Est. Priority Date: 12/20/2012
• Status: Abandoned Application

• Current Assignee: National University Corporation Hokkaido University
• Original Assignee: Lsip, LLC, National University Corporation Hokkaido University
• Inventors: Hatakeyama, Shigetsugu, Tanaka, Shinya
• Application Number:
  US14/653,562
• Publication Number:
  US 20160195535A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 2333/435 from animals; from humans
• CPC Class Codes: G01N 2333/4703 Regulators; Modulating activity
• CPC Class Codes: G01N 2333/4742 Keratin; Cytokeratin
• CPC Class Codes: G01N 2333/4748 p53
• CPC Class Codes: G01N 33/57434 of prostate

Abstract

The purpose of the present invention is to provide a method for detecting prostatic basal cells by searching for molecules that are expressed specifically in prostatic basal cells and analyzing said molecules. The problem can be solved using a method for detecting prostatic basal cells that is characterized by using immunohistochemical staining to visualize the expression of TRIM29 (Tripartite motif-containing protein 29) protein in prostatic basal cells.

Claims

1. A method for detecting prostatic basal cells, comprising visualizing the expression of tripartite motif-containing protein 29 (TRIM29) protein in basal cells of a prostate by immunohistostaining.

2. The method for detecting prostatic basal cells according to claim 1, comprising analyzing the morphology of the glandular tissue structure of the prostate and the visualized expression of tripartite motif-containing protein 29 (TRIM29) protein.

3. The method for detecting prostatic basal cells according to claim 1, further comprising visualizing the expression of cytokeratin and/or p63 protein by immunohistostaining.

4. The method for detecting prostatic basal cells according to claim 3, comprising analyzing the morphology of the glandular tissue structure of the prostate and the visualized expression of cytokeratin protein and/or the visualized expression of p63 protein.

5. A method for identifying the presence, decrease, or disappearance of prostatic basal cells, comprising using the method for detecting prostatic basal cells according to claim 1.

6. A method for diagnosing prostate cancer, comprising a step of detecting prostatic basal cells in prostate tissue collected from a subject by immunohistostaining using an anti-TRIM29 antibody or an antigen-binding fragment thereof.

7. The diagnostic method according to claim 6, wherein the decrease or disappearance of prostatic basal cells as compared to those in normal prostate tissue indicates the presence of prostate cancer.

8-10. -10. (canceled)

11. An immunohistostaining kit for detecting prostatic basal cells, comprising an anti-TRIM29 antibody or an antigen-binding fragment thereof.

12. The immunohistostaining kit for detecting prostatic basal cells according to claim 11, further comprising an anti-cytokeratin antibody or an antigen-binding fragment thereof and/or an anti-p63 antibody or an antigen-binding fragment thereof.