COMPOSITIONS AND METHODS FOR MOLECULAR LABELING
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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Citations
57 Claims
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1-31. -31. (canceled)
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32. A method of obtaining genetic sequence data for assembling a haplotype, the method comprising:
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forming a plurality of compartments, each compartment comprising a polynucleotide template and at least one primer for binding to the polynucleotide template, wherein the primer comprises a barcode and the barcodes differ between compartments; hybridizing the primers to the templates; extending the hybridized primers, whereby a plurality of extended primers comprising barcodes are produced; and determining the nucleic acid sequence of at least a portion of the extended primers, wherein the portion includes the barcodes. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51)
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52. A method of obtaining genetic sequence data for assembling a haplotype, the method comprising:
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forming a plurality of compartments, each compartment comprising a polynucleotide template; attaching a barcode to the polynucleotide template, wherein the barcodes differ between compartments; and determining the nucleic acid sequence of the barcode and at least a portion of the polynucleotide template attached to the barcode. - View Dependent Claims (53, 54, 55, 56, 57)
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Specification