PRODUCTION OF PROBIOTICS BY IN VITRO ENRICHMENT OF BENEFICIAL MICROORGANISMS FROM HUMAN OR ANIMAL MICROBIOTA

• US 20160206668A1
• Filed: 08/22/2014
• Published: 07/21/2016
• Est. Priority Date: 08/22/2013
• Status: Abandoned Application

• Current Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
• Original Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
• Inventors: Kort, Remco, Schuren, Frank Henri Johan
• Application Number:
  US14/912,774
• Publication Number:
  US 20160206668A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

The present invention is directed to a method of treating a dysbiosis in a mammal, preferably a human, by taking a sample of the micro-organisms that are causative of the dysbiosis, whereafter said sample is enriched in vitro for healthy bacteria, after which the sample is replaced.

Claims

1. A method for treatment of a dysbiotic condition in a subject comprising the steps of:

a. obtaining a sample from said subject wherein said sample is obtained from the location of the dysbiotic condition and wherein said sample comprises micro-organisms;

b. in vitro culturing of said sample under conditions that favour beneficial micro-organisms;

c. re-introducing a sample of said cultured micro-organisms into said subject.

5. The method according to claim 1, wherein said subject is a human.

6. The method according to claim 1, wherein said subject is an animal.

7. The method according to claim 1, wherein said location of the dysbiotic condition is selected from the group consisting of intestine, vagina, oral cavity, respiratory tract and skin.

8. The method according to claim 1, wherein the micro-organisms in said sample are bacteria.

9. The method according to claim 1, wherein the beneficial micro-organisms are selected from the group consisting of Lactobacilli, Bifidobacilli and Akkermansia.

10. The method according to claim 1, wherein the in vitro culture is maintained for more than two days.

11. The method according to claim 1, wherein the re-introduction is performed by oral or topical administration.

12. The method according to claim 1, wherein said cultured sample is stored and can be administered to said subject upon recurrence of said dysbiotic condition.

14. The method of claim 6, wherein said animal is selected from the group consisting of cattle, horses, poultry, pigs, dogs and cats.

15. The method of claim 12, wherein the recurrent dysbiotic condition is a recurrent infection.

2. A probiotic composition comprising micro-organisms obtained by culturing a sample comprising micro-organisms obtained from a location of a dysbiotic condition in a subject, wherein the culturing is carried out under conditions that favour beneficial micro-organisms.

3. The probiotic composition according to claim 2, wherein said probiotic composition comprises additional excipients.

4. The probiotic composition according to claim 3, wherein said composition is suitable for administration to the location of the dysbiotic condition.

17. The probiotic composition of claim 2, wherein said subject is a human.

18. The probiotic composition of claim 2, wherein said location of the dysbiotic condition is selected from the group consisting of intestine, vagina, oral cavity, respiratory tract and skin.

19. The probiotic composition of claim 2, wherein the micro-organisms in said sample are bacteria.

20. The probiotic condition of claim 2 wherein the beneficial micro-organisms are selected from the group consisting of Lactobacilli, Bifidobacilli and Akkermansia.

13. A method to prevent the occurrence of an auto-immune disease in children delivered through Caesarean section, comprising:

a. obtaining a vaginal microbiota sample from the mother at the time of delivery, or shortly before or after this time;

b. optionally culturing said sample, during which culture said sample may optionally be enriched for beneficial micro-organisms;

c. administration of at least a part of said sample obtained from step a or step b to the new-born child.

16. The method of claim 13, wherein the administration is oral administration.