COMPOSITIONS AND METHODS USING CAPSIDS RESISTANT TO HYDROLASES

• US 20160208221A1
• Filed: 09/12/2014
• Published: 07/21/2016
• Est. Priority Date: 09/12/2013
• Status: Abandoned Application

• Current Assignee: APSE, LLC (RNAiSSANCE AG LLC)
• Original Assignee: APSE, LLC (RNAiSSANCE AG LLC)
• Inventors: HUANG, Henry, ARHANCET, Juan Pedro Humberto, SUMMERS, Neena
• Application Number:
  US14/914,553
• Publication Number:
  US 20160208221A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12N 15/88 using microencapsulation, e.g. using amphiphile liposome vesicle
• CPC Class Codes: C12N 2795/18122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2795/18123 Virus like particles [VLP]
• CPC Class Codes: C12N 2795/18151 Methods of production or purification of viral material
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.

Claims

1-20. -20. (canceled)

21. A virus-like particle (VLP) comprising a capsid enclosing at least one heterologous cargo molecule and a packing sequence, wherein the capsid is resistant to hydrolysis catalyzed by a category EC 3.4 peptide bond hydrolase.