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Molecular Fabrication

  • US 20160215283A1
  • Filed: 10/02/2013
  • Published: 07/28/2016
  • Est. Priority Date: 12/06/2012
  • Status: Active Grant
First Claim
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1. A method comprising:

  • (a) obtaining;

    (i) a set of origin of replication cassettes;

    (ii) a set of selectable marker cassettes;

    (iii) one or two or more sets of functional cassettes; and

    (iv) a target cassette that comprises a sequence of interest;

    wherein the cassettes in each of (i), (ii), (iii) and (iv) are in different vessels;

    (b) selecting an origin of replication cassette, a selectable marker cassette, and one or two or more functional cassettes from the sets of (a);

    (c) performing an assembly reaction with the cassettes selected in step (b) and said target cassette, wherein said cassettes comprise ends that hybridize with one another to produce a product that can be transformed or transfected into a host cell of interest;

    (d) introducing the product of step (c) into a host cell; and

    (e) selecting a transformed or transfected host cell that harbors a plasmid that comprises said one or two or more selected functional cassettes, said target cassette, said origin of replication cassette and said selectable marker cassette.

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