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PLASMONIC BEADS FOR MULTIPLEXED ANALYSIS BY FLOW DETECTION SYSTEMS

  • US 20160216252A1
  • Filed: 09/11/2014
  • Published: 07/28/2016
  • Est. Priority Date: 09/13/2013
  • Status: Abandoned Application
First Claim
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1. A method for detection of analytes in a sample, comprising:

  • (a) contacting the sample with a population of microparticles in suspension, said microparticles comprising a plasmonically active surface providing near-infrared (“

    NIR”

    ) enhanced fluorescence, said fluorescence optionally provided by gold nano-island covering a portion of said microparticles, said population of microparticles further comprising subpopulations comprising different analyte capture molecules bound to said plasmonically active surface;

    (b) allowing said different analyte capture molecules to form complexes with different analytes that may be present in the sample;

    (c) labeling complexes formed in step (b) with fluorescent labels;

    (d) detecting labeled complexes by irradiating said fluorescent labels and sensing metal enhanced fluorescence from labeled complexes, whereby NIR enhanced fluorescence from said microparticles indicates detection of analytes; and

    (e) distinguishing multiple analytes in the sample, if present, by different NIR enhanced fluorescence signals.

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