METHOD TO ESTIMATE THE AGE OF TISSUES AND CELL TYPES BASED ON EPIGENETIC MARKERS
First Claim
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1. A method for determining the age of a biological sample comprising:
- measuring a methylation level of a set of methylation markers in genomic DNA of the biological sample; and
determining an age of the biological sample with a statistical prediction algorithm, comprising (a) obtaining a linear combination of the methylation marker levels, and (b) applying a transformation to the linear combination to determine the age of the biological sample.
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Abstract
A method for determining the age of a biological sample comprising measuring a methylation level of a set of methylation markers in genomic DNA of the biological sample. An age of the biological sample is determined with a statistical prediction algorithm, comprising (a) obtaining a linear combination of the methylation marker levels, and (b) applying a transformation to the linear combination to determine the age of the biological sample.
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Citations
50 Claims
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1. A method for determining the age of a biological sample comprising:
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measuring a methylation level of a set of methylation markers in genomic DNA of the biological sample; and determining an age of the biological sample with a statistical prediction algorithm, comprising (a) obtaining a linear combination of the methylation marker levels, and (b) applying a transformation to the linear combination to determine the age of the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 30, 31, 32)
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14. A kit comprising probes for detecting methylation markers comprising the CpG positions corresponding to Illumina™
- probe IDs cg22736354, cg09809672, cg21296230, and cg06493994.
- View Dependent Claims (15)
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16. A method for determining an age of a biological sample comprising:
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selectively measuring the methylation levels of a set of methylation markers in genomic DNA of the biological sample, said set of methylation markers comprising markers in at least 6 of the genes listed in Table 3; and determining the age of the sample based on said methylation levels. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 33)
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34. A tangible computer-readable medium comprising computer-readable code that, when executed by a computer, causes the computer to perform operations comprising:
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a) receiving information corresponding to methylation levels of a set of methylation markers in a biological sample, said markers comprising markers in at least 6 of the genes listed in Table 3; and b) determining the age of the biological sample by applying a statistical prediction algorithm to the measured methylation marker levels. - View Dependent Claims (35, 36, 37, 38, 39, 40)
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41. A method for determined the age of an individual comprising:
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collecting a tissue sample from an individual; extracting genomic DNA from the collected tissue sample; measuring a methylation level of a methylation marker on the genomic DNA; and determining an age of the individual with a statistical prediction algorithm, wherein the statistical prediction algorithm is applied to the measured methylation level to determine the age of the individual. - View Dependent Claims (42, 43, 44, 45)
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46. A method for determined the age of the brain of an individual comprising:
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collecting a blood or saliva tissue sample from an individual; extracting genomic DNA from the collected blood or saliva tissue sample; measuring a methylation level of a methylation marker on the genomic DNA, wherein the methylation marker is a CpG methylation marker for a NHLRC1, GREM1, SCGN or EDARADD gene; and determining an age of the brain of the individual with a statistical prediction algorithm, wherein the statistical prediction algorithm is applied to the measured methylation level to determine the age of the individual.
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47. A method for observing the health of an individual comprising:
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collecting a tissue sample from an individual; extracting genomic DNA from the collected tissue sample; measuring a methylation level of a methylation marker on the genomic DNA; determining a biological age of the individual with a statistical prediction algorithm, wherein the statistical prediction algorithm is applied to the measured methylation level to determine the biological age of the individual; and comparing the biological age of the individual to a chronological age of the individual. - View Dependent Claims (48, 49, 50)
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Specification