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METHODS AND DEVICES FOR DE NOVO OLIGONUCLEIC ACID ASSEMBLY

  • US 20160229884A1
  • Filed: 02/03/2016
  • Published: 08/11/2016
  • Est. Priority Date: 02/04/2015
  • Status: Active Grant
First Claim
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1. A method for preparing a surface for oligonucleic acid synthesis, the method comprising:

  • (a) providing a silicon wafer comprising a surface;

    (b) depositing a first molecule on the surface at a first region, wherein the first molecule binds to the surface and lacks a reactive group that binds to a nucleoside phosphoramidite;

    (c) depositing a second molecule on the surface at a second region, wherein the second region comprises a plurality of loci, wherein each locus is surrounded by the first region, and wherein the second molecule binds to the surface and lacks a reactive group that binds to a nucleoside phosphoramidite; and

    (d) depositing a mixture on the surface at the second region, wherein the mixture comprises the second molecule and a third molecule, wherein the third molecule binds to the surface and is capable of binding nucleoside phosphoramidite, and wherein the second molecule and the third molecule are present in the mixture in a molar ratio of 10;

    1 to about 2500;

    1.

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