NANOPARTICLE-MEDIATED GENE DELIVERY, GENOMIC EDITING AND LIGAND-TARGETED MODIFICATION IN VARIOUS CELL POPULATIONS
First Claim
1. A nanoparticle comprising:
- a core polyplex and a silica coating thereon;
wherein said core polyplex comprises an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.
3 Assignments
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Accused Products
Abstract
An improved nanoparticle for transfecting cells is provided. The nanoparticle includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating , where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. Also provided is an improved method of modifying intracellular polynucleotides. The method includes contacting a cell with a nanoparticle that includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.
9 Citations
24 Claims
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1. A nanoparticle comprising:
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a core polyplex and a silica coating thereon; wherein said core polyplex comprises an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of modifying intracellular polynucleotides comprising;
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contacting a cell with a nanoparticle, wherein said nanoparticle comprises a core polyplex and a silica coating thereon; wherein said core polyplex comprises an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification