Parsing Events During MS3 Experiments
First Claim
1. A system for segmenting a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
- a mass spectrometer that performs an MS3 experiment on a sample;
a processor in communication with the mass spectrometer that divides a CID event of the MS3 experiment into two time periods,at the beginning of a first time period of the CID event, instructs the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage, andat the beginning of a second time period of the CID event, instructs the mass spectrometer to both close the pulse valve and apply a second CID voltage, wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time,receives a plurality of second generation fragmentation spectra from the MS3 experiment, andselects second generation fragment ions from the plurality of second generation fragmentation spectra that have an intensity above a threshold intensity level and a signal-to-noise ratio (S/N) above a threshold S/N level for quantitation.
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Abstract
Systems and methods are provided for reducing the time period of a CID event of an MS3 experiment and making the overall fragmentation event more generic. A CID event of an MS3 experiment performed on a sample by a mass spectrometer is divided into two time periods using a processor. At the beginning of a first time period of the CID event, the mass spectrometer is instructed to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage. At the beginning of a second time period of the CID event, the mass spectrometer is instructed to both close the pulse valve and apply a second CID voltage. The mass spectrometer is pumped down during the second time period. The overlap in time of the pump down and CID reduces the overall time period of the CID event.
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Citations
18 Claims
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1. A system for segmenting a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
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a mass spectrometer that performs an MS3 experiment on a sample; a processor in communication with the mass spectrometer that divides a CID event of the MS3 experiment into two time periods, at the beginning of a first time period of the CID event, instructs the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage, and at the beginning of a second time period of the CID event, instructs the mass spectrometer to both close the pulse valve and apply a second CID voltage, wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time, receives a plurality of second generation fragmentation spectra from the MS3 experiment, and selects second generation fragment ions from the plurality of second generation fragmentation spectra that have an intensity above a threshold intensity level and a signal-to-noise ratio (S/N) above a threshold S/N level for quantitation. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for segmenting a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, comprising:
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dividing a CID event of an MS3 experiment performed on a sample by a mass spectrometer into two time periods using a processor; at the beginning of a first time period of the CID event, instructing the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage using the processor; at the beginning of a second time period of the CID event, instructing the mass spectrometer to both close the pulse valve and apply a second CID voltage using the processor, wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time thereby; receiving a plurality of second generation fragmentation spectra from the MS3 experiment using the processor; and selecting second generation fragment ions from the plurality of second generation fragmentation spectra that have an intensity above a threshold intensity level and a signal-to-noise ratio (S/N) above a threshold S/N level for quantitation using the processor. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for segmenting a collision-induced dissociation (CID) event of a mass spectrometry/mass spectrometry/mass spectrometry (MS3) experiment, the method comprising:
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providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise an analysis module and a control module; dividing a CID event of an MS3 experiment performed on a sample by a mass spectrometer into two time periods using the analysis module; at the beginning of a first time period of the CID event, instructing the mass spectrometer to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage using the control module; at the beginning of a second time period of the CID event, instructing the mass spectrometer to both close the pulse valve and apply a second CID voltage using the control module;
wherein the mass spectrometer is pumped down during the second time period allowing pump down and CID to overlap in time;receiving a plurality of second generation fragmentation spectra from the MS3 experiment using the analysis module; and selecting second generation fragment ions from the plurality of second generation fragmentation spectra that have an intensity above a threshold intensity level and a signal-to-noise ratio (S/N) above a threshold S/N level for quantitation using the analysis module. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification