INTRODUCING A LIQUID INTO A DEPRESSION OF A SAMPLE HOLDING PLATE

• US 20160236190A1
• Filed: 02/05/2016
• Published: 08/18/2016
• Est. Priority Date: 02/16/2015
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Marco Kluhr
• Inventors: Kanter, Marcel, Klhr, Marco, Welzel, Oliver Patrick
• Application Number:
  US15/016,754
• Publication Number:
  US 20160236190A1
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2400/021 non contact spotting by inertia, i.e. abrupt deceleration of the nozzle or pin
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced

Abstract

A method is provided for introducing a liquid into a depression of a sample holding plate by way of a controllable dispensing apparatus. The process of the introduction of the liquid is divided into the introduction of a first liquid volume and the introduction of a second liquid volume. A dispensing apparatus is also provided for introducing a liquid into a depression of a sample holding plate. The dispensing apparatus includes a control device that controls an actuator such that a first liquid volume and a second liquid volume are discharged out of an opening of a dispensing head.

Claims

1. A method for introducing a liquid into a depression of a sample holding plate by way of a controllable dispensing apparatus, the method comprising:

discharging a first liquid volume of the liquid out of the dispensing apparatus into the depression at a first speed; and

discharging a second liquid volume of the liquid out of the dispensing apparatus into the depression at a second speed,

wherein the second speed is higher than the first speed.

2. The method of claim 1, wherein the first speed is determined such that the liquid introduced into the depression by the first liquid volume does not, when it impinges upon a wall of the depression, flow out of the depression.

3. The method of claim 2, wherein the first speed is determined based on a size of the first liquid volume, a viscosity of the liquid, a depth of the depression, a geometry of the depression, or a combination thereof.

4. The method of claim 1, wherein the second speed is determined such that the liquid introduced into the depression by the first liquid volume, the second liquid volume, or both the first or second liquid volumes does not flow out of the depression as a result of an impingement of the liquid introduced by the second liquid volume upon the liquid introduced by the first liquid volume.

5. The method of claim 4, wherein the second speed is determined based on a size of the second liquid volume, a viscosity of the liquid, a depth of the depression, a geometry of the depression, or a combination thereof.

6. The method of claim 1, wherein the first speed, the second speed, or both the first speed and the second speed are determined experimentally;

and/or

wherein the first speed, the second speed, or both the first speed and the second speed are calculated based on fluid dynamics equations.

7. The method of claim 6, wherein the first speed, the second speed, or both the first speed and the second speed are determined experimentally based on high-speed images.

8. The method of claim 6, wherein the first speed, the second speed, or both the first speed and the second speed are calculated by way of analysis in accordance with the finite element method.

9. The method of claim 1, further comprising:

waiting for a predefined waiting time between the discharging of the first liquid volume and the discharging of the second liquid volume.

10. The method of claim 9, wherein the predefined waiting time is determined based on a size of the first liquid volume, a size of the second liquid volume, a viscosity of the liquid, a depth of the depression, a geometry of the depression, or a combination thereof.

11. The method of claim 9, wherein the predefined waiting time is determined such that the liquid introduced into the depression by the first liquid volume assumes a state of rest before the second liquid volume is discharged.

12. The method of claim 9, wherein the predefined waiting time is determined such that no separation of the liquid introduced into the depression by the first liquid volume from the liquid introduced into the depression by the second liquid volume occurs.

13. The method of claim 9, wherein the predefined waiting time is determined such that the second liquid volume impinges on the liquid of the first liquid volume as the liquid rises out of the depression after an impingement of the first liquid volume on a wall of the depression.

14. The method of claim 9, wherein the predefined waiting time is determined experimentally;

and/or

wherein the predefined waiting time is calculated on the basis of fluid dynamics equations.

15. The method of claim 14, wherein the predefined waiting time is determined experimentally based on high-speed images.

16. The method of claim 14, wherein the predefined waiting time is calculated by way of analysis in accordance with the finite element method.

17. The method of claim 1, wherein one or more of a maximum acceleration, a maximum variation of the acceleration, or a smoothing factor is predefined for the discharge of the first liquid volume, the second liquid volume, or both the first liquid volume and the second liquid volume.

18. A dispensing apparatus for introducing a liquid into a depression of a sample holding plate, the dispensing apparatus comprising:

a dispensing head with an opening, wherein the dispensing head is designed to accommodate the liquid to be introduced;

an actuator coupled to the dispensing head and designed to discharge the liquid out of the opening of the dispensing head; and

a control device designed to control the actuator such that the actuator discharges a first liquid volume of the liquid out of the opening of the dispensing head into the depression at a first speed and discharges a second liquid volume of the liquid out of the opening of the dispensing head into the depression at a second speed, wherein the second speed is higher than the first speed.

19. The dispensing apparatus of claim 18, wherein the control device is designed to predefine one or more of the first speed, the second speed, or a waiting time between the discharge of the first liquid volume and the discharge of the second liquid volume such that no liquid flows out of the depression as a result of the introduction of the liquid.

20. The dispensing apparatus of claim 19, wherein the control device is designed to predefine one or more of the first speed, the second speed, or the waiting time based on a predefined size of the first liquid volume, a predefined size of the second liquid volume, a viscosity of the liquid, a depth of the depression, a geometry of the depression, or a combination thereof.

21. The dispensing apparatus of claim 19, wherein the control device is further designed to predefine a maximum acceleration, a maximum variation of the acceleration, a smoothing factor, or a combination thereof.