METHODS OF MICROBIAL PRODUCTION OF EXCRETED PRODUCTS FROM METHANE AND RELATED BACTERIAL STRAINS
First Claim
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1. A method for producing at least one excreted product by microbial fermentation of a gaseous substrate, comprising:
- a. providing a gaseous substrate comprising CH4 and optionally, O2, to a culture of at least one methanotrophic microorganism; and
b. maintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 1% of saturation with air to produce at least one excreted product;
ormaintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 40% of saturation with air and reducing respiration to produce at least one excreted product.
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Abstract
The present disclosure is directed to methods of producing excreted products through the fermentation of methane with methanotrophs. In certain embodiments, the methods are performed at low oxygen levels.
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31 Claims
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1. A method for producing at least one excreted product by microbial fermentation of a gaseous substrate, comprising:
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a. providing a gaseous substrate comprising CH4 and optionally, O2, to a culture of at least one methanotrophic microorganism; and b. maintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 1% of saturation with air to produce at least one excreted product;
ormaintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 40% of saturation with air and reducing respiration to produce at least one excreted product. - View Dependent Claims (2, 3, 4, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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5. A method for producing at least one excreted product by microbial fermentation of a gaseous substrate, comprising:
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a. providing a gaseous substrate comprising CH4 and optionally, O2, to a culture of at least one methanotrophic microorganism; and b. maintaining the microorganism under conditions suitable for fermentation to produce at least one excreted product. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14)
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30. An engineered methanotrophic bacterium capable of fermenting methane comprising a deletion of one or more of the following genes:
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a. NADH-ubiquinone oxidoreductase (MALCv4_1304); b. hydrogenase (MALCv4_1307); c. acetate kinase (MALCv4_2853); and d. lactate dehydrogenase (MALCv4_0534); e. NAD-reducing hydrogenase (MALCv4_1304 and
1307);f. bacteriohemelythrin (MALCv4_2316); g. sucrose-phosphate synthase (MALCv4_0614); h. a member of the cytochrome bc1 complex (MALCv4_0634, MALCv4_0633, and MALCv4_0632); i. a glycogen biosynthesis gene (MALCv4_3502;
MALCv4_3503;
MALCv_3504;
MALCv4_3505;
MALCv_3506;
MALCv4_3507, and MALCv_3508); andj. cytochrome aa3 oxidase (MALCv4_2315). - View Dependent Claims (31)
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Specification