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METHOD FOR SELECTING A SINGLE CELL EXPRESSING A HETEROGENEOUS COMBINATION OF ANTIBODIES

  • US 20160238600A1
  • Filed: 04/27/2016
  • Published: 08/18/2016
  • Est. Priority Date: 05/30/2003
  • Status: Active Grant
First Claim
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1. A method for selecting a single recombinant cell that expresses a heterogeneous combination of monospecific and bispecific antibodies, or antibody fragments thereof, wherein the monospecific and bispecific antibodies, or antibody fragments thereof, are human, humanized, or deimmunized, and wherein the heterogeneous combination has specific affinity for two target epitopes, the method comprising:

  • carrying out a process for producing the heterogeneous combination;

    the process comprising providing three different variable regions consisting of two heavy chain regions and one light chain variable region in recombinant cells, wherein antigen binding parts of the variable regions originate from a single species and wherein one variable region is able to functionally pair with more than one other variable region, and under conditions allowing for pairing of variable regions and secretion of the paired regions from the recombinant cells resulting in the production of said heterogeneous combination,providing two target epitopes, andselecting a single recombinant cell from the recombinant cells that produces a heterogeneous combination that binds the two target epitopes,wherein the one light chain variable region comprises a rearranged human immunoglobulin light chain variable region consisting essentially of a germline V and a germline J and encoded by a human immunoglobulin light chain V gene segment joined to a human immunoglobulin light chain J gene segment.

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