HIGH-THROUGHPUT SINGLE-CELL ANALYSIS COMBINING PROTEOMIC AND GENOMIC INFORMATION
First Claim
1. A method of single-cell polynucleotide sequencing, the method comprising:
- enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample;
isolating one or more cells of interest from the enriched cell sample; and
obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells, wherein obtaining sequence information comprises generating a molecularly indexed polynucleotide library from the one or more isolated cells.
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Abstract
Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.
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Citations
20 Claims
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1. A method of single-cell polynucleotide sequencing, the method comprising:
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enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest from the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells, wherein obtaining sequence information comprises generating a molecularly indexed polynucleotide library from the one or more isolated cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification