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SINGLE-CELL NUCLEIC ACIDS FOR HIGH-THROUGHPUT STUDIES

  • US 20160251714A1
  • Filed: 02/26/2016
  • Published: 09/01/2016
  • Est. Priority Date: 02/27/2015
  • Status: Active Grant
First Claim
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1. A method of exposing cells from a population to at least two different reagents, wherein each cell is exposed to the reagents individually, or in groups of two of more, the method comprising:

  • (a) distributing cells from the population to a plurality of capture sites in a microfluidic device so that a plurality of capture sites each comprises one or more cells;

    (b) providing one or more first reagent(s) to each capture site;

    (c) providing one or more second reagent(s) to each capture site, wherein the second reagent(s) is/are different from the first reagent(s) and is/are provided separately from the first reagent(s);

    (d) conducting a reaction, whereby the reaction products encode an item of capture site information;

    (e) recovering the reaction products; and

    (f) analyzing the reaction products, wherein such analysis permits the identification of particular reaction products as having been derived from a single cell or group of cells at a particular capture site.

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