MULTICOMPONENT QUANTITATIVE ANALYSIS METHOD USING CHROMATOGRAPHY

• US 20160266074A1
• Filed: 10/27/2014
• Published: 09/15/2016
• Est. Priority Date: 10/28/2013
• Status: Active Grant

• Current Assignee: Aichi Prefectural Government, Shimadzu Corporation
• Original Assignee: Aichi Prefectural Government, Shimadzu Corporation
• Inventors: UENO, Eiji, KITANO, Riki, MIYAGAWA, Haruhiko
• Granted Patent: US 10,018,602 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01J 20/283 based on silica
• CPC Class Codes: B01J 20/291 Gel sorbents
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: G01N 30/06 Preparation
• CPC Class Codes: G01N 30/7206 interfaced to gas chromatograph interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04
• CPC Class Codes: G01N 30/8665 for calibrating the measuring apparatus
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86

Abstract

A number of analytes are divided into groups based on at least either the elution position determined by silica gel column chromatography under the same condition or the partition ratio in a hexane-acetonitrile partition method. To each group, a compound obtained by labeling one of the compounds in the group by deuterium and/or carbon is assigned as the surrogate. After those surrogates are added as internal standard substances to a standard sample for the creation of calibration curves, a GC/MS analysis for the sample is performed and a calibration curve is created for each analyte. In the measurement of an unknown sample, the same set of surrogates are added to the sample and the GC/MS analysis is performed. A quantitative determination processor determines the quantity of each analyte by comparing a peak area ratio calculated from the analysis result with a calibration curve read from the database.

Claims

1. A multicomponent quantitative analysis method using a chromatograph for determining quantities of a number of compounds contained in a sample using the chromatograph, the method comprising following steps:

at least a portion of a number of analytes which may possibly be contained in a sample to be analyzed is divided into a plurality of groups based on at least either an elution position determined by silica gel column chromatography under a same condition or a partition ratio in a hexane-acetonitrile partition method, and a different surrogate is designated for each group;

the surrogate is added to a standard sample for a creation of calibration curves as a common internal standard substance for analytes included in each group, a chromatographic analysis of the standard sample is performed, and a calibration curve to be used for a quantitative determination of the analytes by an internal standard method is created based on a result of the chromatographic analysis; and

in a quantitative determination of analytes contained in an unknown sample, the plurality of surrogates are added to the unknown sample, a chromatographic analysis of the unknown sample is performed, and a result of this chromatographic analysis is compared with the corresponding calibration curves to determine the quantities of the analytes.

2. The multicomponent quantitative analysis method using a chromatograph according to claim 1, wherein

at least a portion of a number of analytes which may possibly be contained in a sample to be analyzed is divided into a plurality of groups based on relative values of the elution position of each analyte and the elution positions of the surrogates and/or relative values of the partition ratio of each analyte and the partition ratios of the surrogates.

4. The multicomponent quantitative analysis method using a chromatograph according to claim 2, wherein

the surrogate assigned to each group is a compound obtained by a stable-isotope labeling of a compound which is one of the analytes included in that group.

3. The multicomponent quantitative analysis method using a chromatograph according to claim, wherein

the surrogate assigned to each group is a compound obtained by a stable-isotope labeling of a compound which is one of the analytes included in that group.