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NOVEL REAGENTS FOR UNIVERSAL SITE-SPECIFIC LABELING AND MODIFICATIONS OF NUCLEIC ACIDS

  • US 20160272669A1
  • Filed: 03/17/2015
  • Published: 09/22/2016
  • Est. Priority Date: 03/17/2015
  • Status: Active Grant
First Claim
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1. A method for modifying an oligonucleotide, comprising:

  • (a) conjugating H2N(CH2)nNH2 to the 5′

    end of the oligonucleotide to form a conjugated product;

    (b) amidating a free —

    NH2 group of the conjugated product with R1

    N3; and

    (c) reacting the terminal —

    N3 group with one of HC≡

    C—

    R2 and a cycloalkyne, wherein n is an integer from 2 to 6, R1 is a first substituent, and R2 is a second substituent.

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