METHOD AND APPARATUS FOR QUANTITATIVE AND DEPTH RESOLVED HYPERSPECTRAL FLUORESCENCE AND REFLECTANCE IMAGING FOR SURGICAL GUIDANCE
First Claim
1. An optical and image processing system comprising:
- a fluorescent stimulus light source adapted to provide light at a fluorescent stimulus wavelength;
a spatial modulator coupled to modulate light, forming spatially modulated light;
projection apparatus configured to project onto tissue light selected from the group consisting of fluorescent stimulus light and spatially modulated light;
a hyperspectral camera configured to receive light from tissue; and
an image processing system coupled to receive images from the hyperspectral camera and configured with a memory containing machine readable instructions for performing at least one function selected from the group consisting of quantitative fluorescent imaging, and depth resolved fluorescent imaging and for displaying resultant processed fluorescent images.
4 Assignments
0 Petitions
Accused Products
Abstract
An imaging system, such as a surgical microscope, laparoscope, or endoscope or integrated with these devices, includes an illuminator providing patterned white light and/or fluorescent stimulus light. The system receives and images light hyperspectrally, in embodiments using a hyperspectral imaging array, and/or using narrowband tunable filters for passing filtered received light to an imager. Embodiments may construct a 3-D surface model from stereo images, and will estimate optical properties of the target using images taken in patterned light or using other approximations obtained from white light exposures. Hyperspectral images taken under stimulus light are displayed as fluorescent images, and corrected for optical properties of tissue to provide quantitative maps of fluorophore concentration. Spectral information from hyperspectral images is processed to provide depth of fluorophore below the tissue surface. Quantitative images of fluorescence at depth are also prepared. The images are displayed to a surgeon for use in surgery.
58 Citations
21 Claims
-
1. An optical and image processing system comprising:
-
a fluorescent stimulus light source adapted to provide light at a fluorescent stimulus wavelength; a spatial modulator coupled to modulate light, forming spatially modulated light; projection apparatus configured to project onto tissue light selected from the group consisting of fluorescent stimulus light and spatially modulated light; a hyperspectral camera configured to receive light from tissue; and an image processing system coupled to receive images from the hyperspectral camera and configured with a memory containing machine readable instructions for performing at least one function selected from the group consisting of quantitative fluorescent imaging, and depth resolved fluorescent imaging and for displaying resultant processed fluorescent images. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
-
14. A method of imaging tissue comprising:
-
projecting onto tissue spatially modulated fluorescent stimulus light at a fluorescent stimulus wavelength; imaging light from tissue with a hyperspectral camera at at least a first and a second emissions wavelength at each of a plurality of pixels; determining a relationship between depth and ratios of intensity at a first and a second emissions wavelength for a fluorophore in tissue; determining a depth of the fluorophore at each pixel of the plurality of pixels based upon the relationship between depth and the ratios, and the images at the first and the second emissions wavelength; and displaying the depth at each pixel as an image. - View Dependent Claims (15, 16, 17)
-
-
18. A method of imaging tissue comprising:
-
projecting onto tissue spatially modulated white light in at least a first and a second of a plurality of spatially modulated patterns; imaging light from tissue with a hyperspectral camera as illuminated in at least the first and a second spatially modulated pattern to form a plurality greater than three of images each taken in a distinct wavelength band selected from a plurality of wavelength bands, the plurality of wavelengths including more than three wavelength bands and including at least one infrared band; determining optical properties of tissue from the plurality of images, the optical properties of tissue including at least absorbance and reflectance at a first and a second wavelength band of the plurality of wavelength bands; and determining a map of hemoglobin saturation, and hemoglobin concentration in the tissue. - View Dependent Claims (19, 20, 21)
-
Specification