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METHOD FOR BASELINE CORRECTION IN A CHROMATOGRAM

  • US 20160282318A1
  • Filed: 03/10/2016
  • Published: 09/29/2016
  • Est. Priority Date: 03/27/2015
  • Status: Active Grant
First Claim
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1. A method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base, said method comprising the steps of:

  • a) providing a chromatography system with a chromatography column and a UV detector downstream of said chromatography column;

    b) providing UV extinction coefficients at a wavelength λ

    for an acid form and a base form of said at least one UV-absorbing component and a dissociation constant for said component,c) obtaining a chromatogram with said UV detector at wavelength λ

    using said buffer under a set of conditions where pH and/or conductivity is varied between said conditions,d) for a plurality of said conditions calculating the concentrations of said acid and base forms using said dissociation constant and pH and conductivity values, ande) calculating UV absorption values of said acid and base forms from said concentrations and said UV extinction coefficients under said conditions and subtracting said UV absorption values from said chromatogram.

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