Freestanding Ultrathin Membranes and Transfer-Free Fabrication Thereof
First Claim
Patent Images
1. A membrane device comprising:
- (a) a substrate having an upper surface, a lower surface, and an aperture, the aperture having one or more walls connecting the upper and lower surfaces and forming a well; and
(b) a membrane attached to the lower surface of the substrate and forming a floor of the well, the membrane having a thickness of less than 10 nm and an electrical conductance of less than 1 nS/μ
m2.
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Abstract
Devices contain freestanding, ultra thin (<10 nm thick) membranes and methods of making such devices. Methods of using devices contain freestanding ultra thin membranes for determining the sequence of a polynucleotide and for desalination of aqueous solutions. A device containing: a substrate having an upper surface, a lower surface, and an aperture, the aperture having one or more walls connecting the upper and lower surfaces and forming a well; and a membrane attached to the lower surface of the substrate and forming a floor of the well, the membrane having a thickness of less than 10 nm. The electrical conductance across the membrane is less than 1 nS/?m2.
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Citations
92 Claims
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1. A membrane device comprising:
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(a) a substrate having an upper surface, a lower surface, and an aperture, the aperture having one or more walls connecting the upper and lower surfaces and forming a well; and (b) a membrane attached to the lower surface of the substrate and forming a floor of the well, the membrane having a thickness of less than 10 nm and an electrical conductance of less than 1 nS/μ
m2. - View Dependent Claims (3, 15, 16, 18, 20, 38, 39, 40, 41, 42, 43, 44, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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2. A membrane device comprising:
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(a) a substrate having an upper surface, a lower surface, and an aperture, the aperture having one or more walls connecting the upper and lower surfaces and forming a well; (b) a passivating layer coating the lower surface of the substrate; and (c) a membrane attached to the passivating layer and forming a floor of the well, the membrane having a thickness of less than 10 nm and an electrical conductance of less than 1 nS/μ
m2. - View Dependent Claims (17, 19)
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4. A nanopore membrane device comprising:
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(a) a substrate having an upper surface, a lower surface coated with a passivating layer, and an aperture, the aperture having one or more walls connecting the upper and lower surfaces and forming a well; and (b) a membrane attached to the passivating layer and forming a floor of the well, the membrane having a thickness of less than 10 nm and comprising a nanopore, wherein the background conductance of the membrane is less than less than 1 nS/μ
m2. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 45, 46, 47, 48, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 92)
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- 90. The method of 88, wherein the apparatus comprises a primer capable of hybridizing with the polynucleotide.
Specification