Cell Washing Device Using Standing Acoustic Waves And A Phantom Material

• US 20160287778A1
• Filed: 03/31/2015
• Published: 10/06/2016
• Est. Priority Date: 03/31/2015
• Status: Active Grant

• Current Assignee: Biomet Manufacturing, LLC (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Biomet Biologics LLC (Zimmer Biomet Holdings, Inc.)
• Inventors: CUNNINGHAM, Grant D., KING, Scott R., LEACH, Michael D., HAMMAN, Ned M., ABESKARON, David, DORIAN, Randel, STORRS, Richard W.
• Granted Patent: US 9,855,382 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42
• CPC Class Codes: A61M 1/0281 Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
• CPC Class Codes: A61M 1/3678 Separation of cells using wave pressure; Manipulation of individual corpuscles
• CPC Class Codes: A61M 1/3692 Washing or rinsing blood or blood constituents
• CPC Class Codes: A61M 2202/0429 Red blood cells; Erythrocytes
• CPC Class Codes: A61M 2205/3375 Acoustical, e.g. ultrasonic, measuring means
• CPC Class Codes: B01D 21/283 Settling tanks provided with vibrators
• CPC Class Codes: B01D 43/00 Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration flotation processes B03D1/00; drying solid materials or objects F26B
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 5/0641 Erythrocytes

Abstract

Devices for washing a composition including cells are provided. The devices include a separation channel, a first wave component and a second wave component. The separation channel is positioned between the first and second wave components. Collectively, the wave components generate a standing bulk acoustic wave that isolates cells from the composition.

Claims

12. A device for washing a multicomponent mixture, the device comprising:

a body having a first surface, and a second opposing surface

a first wave generator coupled to the first surface; and

a wave component selected from the group consisting of a second wave generator, a first reflective material, a first reflective surface, and a first reflective layer, wherein the wave component is coupled to or forms the second surface,

wherein the body defines a channel having a channel floor, channel walls, and a channel ceiling, the channel extending along a longitudinal axis from a first end region of the device to a second end region of the device, the channel comprising a receiving region near the first end region, a collection region near the second end region, and a separation region between the receiving region and collection region, the separation region being positioned between the first wave generator and the wave component; and

wherein the channel floor and walls comprise a phantom material.

13. The device according to claim 12, wherein the first wave generator and the wave component generate a standing bulk acoustic wave with a pressure node located in the separation region of the channel.

14. The device according to claim 12, further comprising:

a first side;

a second opposing side;

a third wave generator coupled to the first side; and

a second wave component selected from the group consisting of a fourth wave generator, a second reflective material, a second reflective surface, and a second reflective layer, wherein the second wave component is coupled to the second side.

15. The device according to claim 14, wherein the first wave generator and wave component generate a first standing acoustic wave with a first pressure node located in the separation region of the channel, and the third wave generator and the second wave component generate a second standing acoustic wave with a second pressure node located in the separation region of the channel, wherein the first standing acoustic wave is orthogonal to the second standing acoustic wave.

16. The device according to claim 12, further comprising a first inlet and a second inlet near the first end region and in fluid communication with the mixing region of the channel, and a first outlet and a second outlet positioned near the second end region and in fluid communication with the collection region of the channel.

20. The device according to claim 12, wherein the phantom material mimics the acoustic properties of water.

21. The device according to claim 12, wherein the separation channel has a length of from about 20 mm to about 100 mm, a width from about 5 mm to about 50 mm, and a height of from about 0.5 mm to about 3 mm.

22. The device according to claim 12, wherein the body defines a first planar shelf that bifurcates the receiving region of the channel and a second planar shelf that bifurcates the collection region of the channel.

23. The device according to claim 22, wherein the channel has a rectangular cross-sectional geometry.

24. The device according to claim 23, where the device is composed of a plurality of layers that are bonded together by an adhesive.

17-1. The device according to claim 17, wherein the first wave generator and the second wave generator or first reflective material generate a standing bulk acoustic wave with a pressure node positioned in the separation region of the channel, and the pressure node separates the red blood cells from the wash solution.

19. The device according to claim 18, wherein the red blood cells exit the device through the first outlet and the wash material exits the device through the second outlet.

25. A system for washing a multicomponent mixture, the system comprising:

a base unit comprising a first wave component and at least one coupling member; and

a disposable separation device comprising a body having a first surface, and a second opposing surface, wherein the first surface comprises a second wave component and the second surface optionally comprises a third wave component, and wherein the body defines a channel extending along a longitudinal axis from a first end region of the device to a second end region of the device, the channel comprising a receiving region near the first end region, a collection region near the second end region and a separation region between the second wave component and the optional third wave component,

wherein the coupling member is configured to couple the disposable separation device to the base unit such that the separation region of the channel is positioned between the first wave component of the base unit and the second wave component of the disposable separation device.

26. The system according to claim 25, wherein the first wave component is a wave generator, the second wave component is selected from the group consisting of a second wave generator, a reflective material, a reflective surface, and a reflective layer, and the device does not comprises a third wave component.

27. The system according to claim 26, wherein the wave generator and second wave component generate a standing acoustic wave with a pressure node located in the separation region of the channel.

28. The system according to claim 25, wherein the first wave component is an electrical contact, the third wave component is a wave generator, and the second wave component is selected from the group consisting of a second wave generator, a reflective material, a reflective surface, and a reflective layer.

29. The system according to claim 28, wherein the wave generator in is direct contact with the electrical contact and the wave generator and the second wave component generate a standing acoustic wave with a pressure node located in the separation region of the channel.

30. The system according to claim 25, wherein the body of the disposable separation device defines a first planar shelf that bifurcates the receiving region of the channel and a second planar shelf that bifurcates the collection region of the channel.

31. A method for washing a composition comprising red blood cells, the method comprising:

delivering the composition comprising red blood cells and a wash material into a separation device comprising a body having a first surface including a first wave component and a second opposing surface having a second wave component, the body defining a separation channel extending from a first end region of the device to a second end region of the device, the channel having a receiving region, a separation region and an collection region;

generating a standing acoustic wave with a pressure node located in the separation region of the channel, wherein the standing acoustic wave is generated by the first and second wave components;

isolating red blood cells by passing the composition comprising red blood cells and wash material relative to the pressure node, wherein the pressure node forces the red blood cells to the collection region of the channel; and

collecting the red blood cells at an outlet of the device that is in fluid communication with the collection region of the channel.