DEVICES AND SYSTEMS FOR MOLECULAR BARCODING OF NUCLEIC ACID TARGETS IN SINGLE CELLS
1 Assignment
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Accused Products
Abstract
Disclosed herein are devices and systems comprising a) a substrate comprising at least 100 microwells and a plurality of beads, wherein a plurality of the at least 100 microwells each contain a single bead, and wherein the ratio of the average diameter of the microwells to the diameter of the beads ranges from about 1.2 to about 1.8; b) a flow cell in fluid communication with the substrate; and c) at least one inlet port and at least one outlet port, wherein the at least one inlet port and at least one outlet port are capable of directing a flow of a fluid through the flow cell, thereby contacting the microwells with the fluid.
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Citations
116 Claims
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1. (canceled)
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2. A device comprising:
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a substrate comprising at least 100 microwells, wherein each microwell has a volume ranging from about 1,000 μ
m3 to about 786,000 μ
m3, and wherein a surface of the at least 100 microwells is coated with a surface coating to improve wettability; anda flow cell in fluid communication with the substrate. - View Dependent Claims (7, 9, 11, 13, 14, 16, 17, 18, 20, 21, 22, 24, 26, 27, 33, 36, 40, 41, 42, 44, 45)
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3. (canceled)
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46-51. -51. (canceled)
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52. A system comprising:
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a device comprising; a substrate comprising at least 100 microwells, wherein each microwell has a volume ranging from about 1,000 μ
m3 to about 786,000 μ
m3, and wherein a surface of the at least 100 microwells is coated with a surface coating to improve wettability;a flow cell in fluid communication with the substrate; and at least one inlet port and at least one outlet port, wherein the at least one inlet port and at least one outlet port are capable of directing a flow of a fluid through the flow cell, thereby contacting the microwells with the fluid; and a flow controller;
wherein the flow controller is configured to control the delivery of fluids. - View Dependent Claims (54, 56, 57, 58, 59)
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53. (canceled)
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60-113. -113. (canceled)
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114. A method for loading one or more cell samples into microwells of a device, wherein the device comprises:
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a substrate comprising at least 100 microwells, wherein each microwell has a volume ranging from about 1,000 μ
m3 to about 786,000 μ
m3, and wherein a surface of the at least 100 microwells is coated with a surface coating to improve wettability; anda flow cell in fluid communication with the substrate, the method comprising; injecting air into the flow cell in fluid communication with the substrate comprising at least 100 microwells; injecting a cell sample into the flow cell; and injecting air into the flow cell. - View Dependent Claims (115)
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116-163. -163. (canceled)
Specification