COMPOSITIONS AND METHODS FOR MOLECULAR LABELING
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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Citations
77 Claims
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1-54. -54. (canceled)
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55. A method for barcoding RNA from a single cell, the method comprising:
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introducing a plurality of nucleic acid constructs into a fluid partition comprising a plurality of RNA molecules from a single cell, wherein each of the nucleic acid constructs comprises a barcode correlated with the cell; hybridizing at least one nucleic acid construct to at least one RNA molecule; and producing at least one barcoded RNA complex. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71)
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72. A method for barcoding a target sequence from a single cell, the method comprising:
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introducing a plurality of nucleic acid constructs into a plurality of fluid partitions each partition comprising a plurality of target sequences from a single cell, wherein each of the nucleic acid constructs within the same fluid partition comprises a barcode sequence correlated with the cell; hybridizing at least one nucleic acid construct to the target sequence in a fluid partition; and producing a barcoded target complex in the fluid partition. - View Dependent Claims (73, 74, 75, 76, 77)
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Specification