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OPTIMIZED SMALL GUIDE RNAS AND METHODS OF USE

  • US 20160289673A1
  • Filed: 09/29/2014
  • Published: 10/06/2016
  • Est. Priority Date: 09/27/2013
  • Status: Active Grant
First Claim
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1. A small guide RNA molecule comprising from 5′

  • to 3′

    ;

    a binding region, comprising between about 5 and about 50 nucleotides;

    a 5′

    hairpin region, comprising;

    fewer than four consecutive uracil nucleotides;

    ora length of at least 31 nucleotides; and

    a 3′

    hairpin region; and

    a transcription termination sequence,wherein the small guide RNA is configured to form a complex with a small guide RNA-mediated nuclease, the complex having increased stability or activity relative to a complex containing a small guide RNA-mediated nuclease and a small guide RNA comprising at least 95% identity to SEQ ID NO;

    1 or a complement thereof.

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